NT21MP negatively regulates paclitaxel-resistant cells by targeting miR‑155‑3p and miR‑155-5p via the CXCR4 pathway in breast cancer

International Journal of Oncology
Yueyue WangQingling Yang

Abstract

Evidence has shown that microRNAs (miRNAs) are vital in cell growth, migration, and invasion by inhibiting their target genes. A previous study demonstrated that miRNA (miR)-155‑3p and miR‑155-5p exerted opposite effects on cell proliferation, apoptosis, migration and invasion in breast cancer cell lines. An miRNA microarray was used to show that miR‑155‑3p was downregulated whereas miR‑155-5p was upregulated in paclitaxel-resistant (PR) cells compared with parental breast cancer cells. However, the role of miR‑155 in breast cancer cell invasion and metastasis remains to be elucidated. A 21-residue peptide derived from the viral macrophage inflammatory protein II (NT21MP), competes with the ligand of CXC chemokine receptor 4 (CXCR4) and its ligand stromal cell-derived factor-1α, inducing cell apoptosis in breast cancer. The present study aimed to identify the underlying mechanism of action of miR‑155‑3p/5p and NT21MP in PR breast cancer cells. Quantitative polymerase chain reaction, western blotting, wound-healing, cell cycle and apoptosis assays, and Cell Counting kit-8 assay were used to achieve this goal. The combined overexpression of miR‑155‑3p with NT21MP decreased the migration and invasion ability and increased the numbe...Continue Reading

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Methods Mentioned

BETA
transfections
transfection
PCR
protein assay
electrophoresis
flow cytometry

Software Mentioned

Image J
miRanda
TargetScan
Bio
miRTarbase
SPSS
ImageJ
Rad Image - Lab

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