NTP pattern of avian embryonic red cells: role of RNA degradation and AMP deaminase/5'-nucleotidase activity

American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
R BaumannS Dragon


During terminal erythroid differentiation, degradation of RNA is a potential source for nucleotide triphosphates (NTPs) that act as allosteric effectors of hemoglobin. In this investigation, we assessed the developmental profile of RNA and purine/pyrimidine trinucleotides in circulating embryonic chick red blood cells (RBC). Extensive changes of the NTP pattern are observed which differ significantly from what is observed for adult RBC. The biochemical mechanisms have not been identified yet. Therefore, we studied the role of AMP deaminase and IMP/GMP 5'-nucleotidase, which are key enzymes for the regulation of the purine nucleotide pool. Finally, we tested the effect of major NTPs on the oxygen affinity of embryonic/adult hemoglobin. The results are as follows. 1) Together with ATP, UTP and CTP serve as allosteric effectors of hemoglobin. 2) Degradation of erythroid RNA is apparently a major source for NTPs. 3) Developmental changes of nucleotide content depend on the activities of key enzymes (AMP deaminase, IMP/GMP 5'-nucleotidase, and pyrimidine 5'-nucleotidase). 4) Oxygen-dependent hormonal regulation of AMP deaminase adjusts the red cell ATP concentration and therefore the hemoglobin oxygen affinity.


Jun 20, 2007·Biochemical and Biophysical Research Communications·Simone K SteinerS Dragon
Nov 12, 2005·European Journal of Clinical Investigation·R Baumann, S Dragon
Jun 30, 2017·Frontiers in Immunology·Sameer A Barghouthi


Mar 1, 1976·Journal of Applied Physiology·H TazawaM Mochizuki
Jan 1, 1976·Comparative Biochemistry and Physiology. A, Comparative Physiology·G R Bartlett, T A Borgese
Mar 25, 1975·Biochemical Pharmacology·R P AgarwalR E Parks
Aug 1, 1975·Science·R E Isaacks, D R Harkness
Oct 1, 1992·Physiological Reviews·R Baumann, H J Meuer
Nov 15, 1991·Archives of Biochemistry and Biophysics·B HuC M Hohl
Nov 1, 1991·The American Journal of Physiology·D MillionR Baumann
Jan 1, 1986·Human Heredity·G NovelliB Dallapiccola
Mar 1, 1986·The Journal of Clinical Investigation·F BontempsH G Hers
Oct 25, 1973·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·G A Bruns, V M Ingram
May 1, 1983·The American Journal of Physiology·R BaumannT Brilmayer
Apr 1, 1983·Respiration Physiology·G N Lapennas, R B Reeves
Dec 1, 1982·Journal of Applied Physiology: Respiratory, Environmental and Exercise Physiology·R BaumannE A Haller
Mar 1, 1995·Trends in Genetics : TIG·A J Copp
Nov 9, 1994·Molecular and Cellular Biochemistry·T W Traut
Mar 10, 1999·The American Journal of Physiology·C M SatterwhiteM E Bradley
Apr 6, 2000·American Journal of Physiology. Heart and Circulatory Physiology·H H DietrichR G Dacey
Jun 5, 2001·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·World Medical Association General Assembly.
Jul 18, 2002·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·World Medical Association, American Physiological Society

Related Concepts

Hormone Regulation
RNA Degradation
Enzymes, antithrombotic
Biochemical Mechanism
Purine Nucleotides
Red blood cells, blood product
Cell Differentiation Process

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