PMID: 4294Jan 1, 1975

Nuclear 3alpha-hydroxysteroid dehydrogenase (3alphaOHD) activity for 5alpha-dihydrotestosterone in the rat prostate

Endocrine Research Communications
E J Van DoornA F Clark


The in vitro examination of adult male rat prostatic 3alpha-hydroxysteroid dehydrogenase (3alphaOHD) activity using 5alpha-dihydrotestosterone4 as substrate indicates that significant levels of enzyme activity are associated with purified nuclei as well as with the cytosol fractions. Both the purified nuclear and the cytosol fractions exhibited higher levels of 3alphaOHD activity with NADH than with NADPH. The pH activity curves for the NADH and NADPH catalyzed reactions were different for both the nuclear and cytosol fractions. The results suggest the presence of a number of 3alphaOHD enzymes in rat prostate.


Nov 1, 1970·The Biochemical Journal·W B PeelingK Griffiths
Jun 1, 1966·The Biochemical Journal·J ChamberlainP Ofner
Feb 1, 1972·Endocrinologia Japonica·J ShimazakiK Shida
Jul 1, 1973·Acta Endocrinologica·K Nozu, B Tamaoki
Jun 1, 1971·Acta Endocrinologica·V HanssonA Attramadal
Jul 29, 1969·Biochimica Et Biophysica Acta·J E BelhamD C Williams

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