Nuclear accumulation of HMG2 protein is mediated by basic regions interspaced with a long DNA-binding sequence, and retention within the nucleus requires the acidic carboxyl terminus

Biochemistry
H ShirakawaM Yoshida

Abstract

High mobility group 2 (HMG2) protein is ubiquitously distributed in the nucleus of higher eukaryotic cells. Accumulation of an HMG2-beta-galactosidase fusion protein expressed in COS-7 cells suggested active transport of HMG2 protein into the nucleus after translation in the cytoplasm. Deletion analysis of the HMG2 sequence in the HMG2-beta-galactosidase fusion protein indicated that basic regions interspaced with the long DNA-binding sequence in HMG2, called the HMG1/2 box, are necessary for the nuclear accumulation of HMG2. The close configuration of basic regions at both ends of the DNA-binding sequence in the tertiary structure may function as the nuclear localization signal. This novel nuclear localization signal structure is different from typical ones such as the single or bipartite basic cluster in many nuclear proteins. A portion of the HMG2 molecule remained in the cytoplasm after translation. Interspecies heterokaryon assay demonstrated that the acidic carboxyl terminus of HMG2 was necessary for retention of the protein in the nucleus.

References

Nov 6, 1989·European Journal of Biochemistry·M I MosevitskyM A Zabezhinsky
Feb 1, 1989·The Journal of Cell Biology·M Kozak
Jan 1, 1982·Journal of Gerontology·M MatsuoK Hosoda
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Citations

Jul 5, 2001·Molecular and Cellular Biology·M Prymakowska-BosakM Bustin
Feb 24, 2009·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Gabi GerlitzMichael Bustin
Sep 12, 2006·Experimental Cell Research·George HoppeJonathan E Sears
Nov 23, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ju Ho Youn, Jeon-Soo Shin
Oct 25, 2002·American Journal of Physiology. Cell Physiology·Paul A RobinsonChristina A Mitchell

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