PMID: 9427284Jan 14, 1998Paper

Nuclear accumulation of Saccharomyces cerevisiae Mcm3 is dependent on its nuclear localization sequence

Genes to Cells : Devoted to Molecular & Cellular Mechanisms
M R YoungBik K Tye

Abstract

The proteins of the Mcm2-7 family are required for the initiation of DNA replication. In Saccharomyces cerevisiae the nuclear envelope does not break down during the mitotic phase of the cell cycle. Large nuclear proteins, such as the Mcm proteins, which accumulate in the nucleus during specific portions of the cell cycle, must have regulated mechanisms to direct their entry into the nucleus. We have identified a nuclear localization sequence (NLS) in Mcm3, and demonstrated that it is necessary for the translocation of Mcm3 into the nucleus and sufficient for directing Escherichia coli beta-galactosidase to the nucleus. Immediately adjacent to the nuclear localization sequence are four potential sites for phosphorylation by Cdc28. Mutagenesis of all four sites has no immediate phenotypic effect on cell growth or viability, nor does it affect nuclear accumulation of Mcm3, although two-dimensional protein gel analysis has shown that at least some of these sites are normally phosphorylated in vivo. Substitution of the Mcm3 NLS by the SV40 large T-antigen NLS also directs the nuclear accumulation of the Mcm3-T-antigen protein, although cell growth is compromised. Replication activity in cells bearing either the Mcm3-Cdc28 phosphory...Continue Reading

Citations

Dec 9, 2014·Cell Cycle·Minori ShinyaSatoru Mimura
Jun 16, 2001·The Journal of Biological Chemistry·A A KulkarniT G Cooper
Sep 13, 2002·The Journal of Biological Chemistry·Yoshinori TakeiRonald Laskey
Dec 11, 2002·The Journal of Biological Chemistry·Victoria K ChangBik K Tye
Sep 25, 2001·The Journal of Biological Chemistry·Y IshimiH Masai
Jun 29, 2000·Annual Review of Biochemistry·B K Tye
Sep 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·Haiyan PanJeffrey W Pollard
Nov 24, 1999·Nature Cell Biology·J J Blow, T A Prokhorova
Dec 10, 1999·Molecular Biology of the Cell·S G Pasion, S L Forsburg
Mar 10, 2004·Microbiology and Molecular Biology Reviews : MMBR·Susan L Forsburg
Aug 24, 1999·Methods : a Companion to Methods in Enzymology·B K Tye
Aug 5, 1998·Biochimica Et Biophysica Acta·S E Kearsey, K Labib
May 17, 2008·DNA Repair·Marta B Davidson, Grant W Brown

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