Nuclear accumulation of the AT1 receptor in a rat vascular smooth muscle cell line: effects upon signal transduction and cellular proliferation

Journal of Molecular and Cellular Cardiology
J L CookRichard N Re

Abstract

The objective of the study was to identify the functional outcome of intracellular versus extracellular angiotensin II-AT(1) receptor interactions in vascular cells. Rat vascular smooth muscle cell line A10 was transfected, independently and concurrently, with plasmids encoding fluorescent fusion proteins of rat angiotensin II (pECFP/AII, encodes AII fused downstream of enhanced cyan fluorescent protein) and the rat AT(1a) receptor (pAT(1)R/EYFP, encodes the rat AT(1a) receptor fused upstream of enhanced yellow fluorescent protein). The AII fluorescent fusion protein possesses no secretory signal peptide and deconvolution microscopy established that is maintained within these cells predominantly in the nucleus. AT(1)R/EYFP was absent from the nucleus when expressed exclusively or in untreated cells but accumulated in the nucleus following exogenous AII treatment or when co-expressed with ECFP/AII. Furthermore, expression of ECFP/AII stimulated proliferation of A10 vascular smooth muscle cells (VSMCs) 1.6-fold (P < 0.05). Transfection of a control, pECFP/AII(C) (which encodes a scrambled AII peptide fused to ECFP) had no growth effect. In light of the intracellular growth effects of ECFP/AII, we sought to elucidate the underlyin...Continue Reading

Citations

May 10, 2007·Journal of the Renin-angiotensin-aldosterone System : JRAAS·Jia L Zhuo, Xiao C Li
Aug 5, 2011·American Journal of Physiology. Cell Physiology·Julia L CookRichard N Re
Feb 22, 2013·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Xiao C Li, Jia L Zhuo
Oct 24, 2007·Naunyn-Schmiedeberg's Archives of Pharmacology·Adam Holownia, Jan J Braszko
Nov 30, 2014·The Journal of Physiology·Artavazd TadevosyanStanley Nattel
May 12, 2009·The Medical Clinics of North America·Richard N Re
May 7, 2009·Molecular and Cellular Endocrinology·John HuynhLeonard Keith Pattenden
Jan 18, 2008·Chemico-biological Interactions·Li-Ping JiangHong-Zhuan Chen
May 19, 2007·Trends in Endocrinology and Metabolism : TEM·Rajesh KumarKenneth M Baker
Dec 2, 2008·Liver International : Official Journal of the International Association for the Study of the Liver·Sigrid SchulteHans-Michael Steffen
May 18, 2012·Clinical Science·Rajesh KumarKenneth M Baker
Jun 13, 2013·Journal of Molecular and Cellular Cardiology·Clémence MerlenBruce G Allen
Dec 30, 2014·Journal of the American Society of Hypertension : JASH·Richard N Re
May 31, 2016·The American Journal of the Medical Sciences·Richard N Re
Aug 5, 2017·Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquée, Nutrition Et Métabolisme·Maja BundaloAleksandra Stanković
Sep 26, 2007·Nature Clinical Practice. Cardiovascular Medicine·Richard N Re, Julia L Cook
Nov 24, 2006·American Journal of Physiology. Cell Physiology·Julia L CookRichard N Re
May 29, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Richard Re
Jan 29, 2008·Journal of Clinical Pharmacology·Richard N Re, Julia L Cook
Apr 26, 2008·Journal of Receptor and Signal Transduction Research·Benoit BoivinTerence E Hébert
Dec 16, 2011·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Brianne EllisJia L Zhuo
Dec 16, 2011·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Julia L Cook, Richard N Re
Dec 16, 2011·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Rajesh KumarKenneth M Baker
Dec 16, 2011·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·TanYa M GwathmeyMark C Chappell
Aug 28, 2020·Frontiers in Pharmacology·Natalia L Rukavina MikusicMariela M Gironacci
Oct 12, 2020·Progress in Neurobiology·Jose L Labandeira-GarciaAna I Rodriguez-Perez

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