Nuclear accumulation of ZFP36L1 is cell cycle-dependent and determined by a C-terminal serine-rich cluster.

Journal of Biochemistry
Yuki MatsuuraHiroyuki Kawahara

Abstract

ZFP36L1 is an RNA-binding protein responsible for mRNA decay in the cytoplasm. ZFP36L1 has also been suggested as a nuclear-cytoplasmic shuttling protein because it contains a potential nuclear localization signal and a nuclear export signal. However, it remains unclear how the nuclear localization of ZFP36L1 is controlled. In this study, we provide evidence that the nuclear accumulation of ZFP36L1 protein is modulated in a cell cycle-dependent manner. ZFP36L1 protein accumulation in fractionated nuclei was particularly prominent in cells arrested at G1-/S-phase boundary, while it was downregulated in S-phase cells, and eventually disappeared in G2-phase nuclei. Moreover, forced nuclear targeting of ZFP36L1 revealed marked downregulation of this protein in S- and G2-phase cells, suggesting that ZFP36L1 can be eliminated in the nucleus. The C-terminal serine-rich cluster of ZFP36L1 is critical for the regulation of its nuclear accumulation because truncation of this probable disordered region enhanced the nuclear localization of ZFP36L1, increased its stability and abolished its cell cycle-dependent fluctuations. These findings provide the first hints to the question of how ZFP36L1 nuclear accumulation is controlled during the c...Continue Reading

References

Mar 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·D CaputA Cerami
Apr 1, 1995·Molecular and Cellular Biology·A M ZubiagaM E Greenberg
Jan 18, 2002·The Journal of Biological Chemistry·Ruth S PhillipsPerry J Blackshear
Jun 12, 2002·Molecular Biology of the Cell·Michael L WhitfieldDavid Botstein
Nov 21, 2002·Biochemical Society Transactions·P J Blackshear
Feb 26, 2004·Nature Structural & Molecular Biology·Brian P HudsonPeter E Wright
Nov 13, 2004·The EMBO Journal·Martin SchmidlinChristoph Moroni
Aug 3, 2005·The Journal of Biological Chemistry·Wi S LaiPerry J Blackshear
Nov 16, 2005·Annual Review of Genetics·Jürg Bähler
Jan 5, 2006·Nucleic Acids Research·Carine BarreauH Beverley Osborne
Nov 30, 2006·Journal of Cellular Biochemistry·Heidi H HauPaul R Bohjanen
Mar 7, 2012·Nature Reviews. Genetics·Daniel R Schoenberg, Lynne E Maquat
Feb 23, 2013·Biochimica Et Biophysica Acta·Seth A Brooks, Perry J Blackshear
Jul 24, 2013·Nature Reviews. Molecular Cell Biology·Cosetta BertoliRobertus A M de Bruin
Aug 21, 2013·PloS One·Laure TwyffelsVéronique Kruys
Aug 12, 2014·Nucleic Acids Research·Shungo AdachiTohru Natsume
Apr 23, 2016·Science·Alison GallowayMartin Turner
Aug 28, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Katharina U VogelMartin Turner
Oct 7, 2016·Molecular Biology of the Cell·Felicitas RatajNadia Cherradi
May 2, 2018·Biochemical and Biophysical Research Communications·Makoto KondoHiroyuki Kawahara

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