Nuclear and cytoplasmic localization of the beta-amyloid peptide (1-43) in transfected 293 cells

Biochemical and Biophysical Research Communications
E M JohnstoneS P Little

Abstract

Cultures of transformed human embryonic kidney 293 cells were transiently transfected with minigene constructs coding for the Abeta peptide (1-43). The Abeta minigene used in this study consisted of exons 16 and 17 of the amyloid precursor protein gene, including the 6000+ bp intronic region. Two of the constructs used in this study, human amyloid precursor protein (APP) promoter-driven Abeta minigene and BK virus enhancer/adenovirus major late promoter-driven Abeta minigene, did not contain a signal peptide sequence, whereas the third, human APP promoter-signal peptide Abeta minigene did not contain the human APP signal sequence. The resulting Abeta products were detected by immune precipitation, using 10D5 antibody and Western blot analysis, using R1280 antisera, as SDS stable oligomers in cell lysates of cells containing all three constructs or in culture media when produced by the signal peptide construct. Evaluation of the cells by immunocytochemistry using conventional and transmission electron microscopy indicated that the cells transfected with constructs without the signal peptide accumulated immunoreactive Abeta primarily in the nucleus.

Citations

Dec 6, 2011·The Journal of Biological Chemistry·Alessio CardinaleDaniela Merlo
May 29, 2008·The Journal of Biological Chemistry·Robert P FriedlandMichael G Zagorski
Mar 7, 2001·Biological Psychiatry·H ZengM G Zagorski
Mar 7, 2006·The Journal of Biological Chemistry·Kenneth M RosenHenry W Querfurth
Nov 19, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yasumasa OhyagiTakeshi Tabira
Aug 29, 2016·Journal of Alzheimer's Disease : JAD·Svetlana A KhmelevaAlexander A Makarov
Aug 1, 1998·Amyloid : the International Journal of Experimental and Clinical Investigation : the Official Journal of the International Society of Amyloidosis·J G CulvenorR Cappai
Dec 17, 1997·Biochemical and Biophysical Research Communications·N W FoxS P Little
Jan 8, 2015·Journal of the American Chemical Society·Jiasi WangXiaogang Qu
Feb 5, 2014·Experimental Cell Research·Ibolya E András, Michal Toborek

❮ Previous
Next ❯

Related Concepts

Related Feeds

Alzheimer's Disease: APP

Amyloid precursor protein (APP) proteolysis is critical for the development of Alzheimer's disease, a neurodegenerative disease associated with accumulation of amyloid plaques in the brain. Here is the latest research on APP and Alzheimer's disease.

BK Virus Infection

BK virus infection is a significant complication of modern immunosuppression used in kidney transplantation. Discover the latest research on BK virus infection here.

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.

© 2021 Meta ULC. All rights reserved