Nuclear and cytosolic calcium signaling induced by extracellular ATP in rat kidney inner medullary collecting duct cells

Biochemical and Biophysical Research Communications
Y SukoY Masuda

Abstract

Using a laser scanning confocal microscopy of fluorescent Ca2+ indicator (Fluo-3-AM) the spatiotemporal Ca2+ dynamics in cultured kidney inner medullary collecting duct cells was investigated. In response to extracellular ATP (100 microM), nuclear (Fln) and cytosolic (Flc) fluorescence intensity increased simultaneously. UTP similarly increased Fln and Flc, but ADP and AMP did not. A ratio between Fln and Flc was about 1.06 +/- 0.03 at rest and increased 1.71 +/- 0.02 at the peak of stimulation (n = 74). In Ca(2+)-free condition, ATP increased Fln and Flc with a smaller peak intensity, but the peak ratio was similar (1.52 +/- 0.03, n = 70). Faster time resolution of 100 ms in line scanning mode did not detect the delay between nuclear and cytosolic Ca2- responses. Our results indicate that nuclear Ca2+ was not diffused from the cytoplasm and that it may be directly released from the nuclear envelope, a possible Ca2+ store.

References

Aug 14, 1992·Biochimica Et Biophysica Acta·O BachsE Carafoli
Feb 1, 1991·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·D A PrzywaraA R Wakade
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·A N MalviyaG Vincendon
Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·P NicoteraP O Berggren
Apr 1, 1986·The Journal of Cell Biology·I LangR Peters
May 1, 1989·The American Journal of Physiology·J M WeinbergP R Knight
Aug 1, 1989·The Journal of General Physiology·M Iino
Jan 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·P NicoteraS Orrenius
Sep 14, 1995·Biochemical and Biophysical Research Communications·J Gasalla-HerraizC M Isales
Dec 20, 1994·Proceedings of the National Academy of Sciences of the United States of America·N L AllbrittonT Meyer
Aug 1, 1993·Endocrine Journal·S Ishikawa
Feb 24, 1994·Nature·F A al-MohannaS R Bolsover
Aug 1, 1994·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·B HimpensM Bollen
Sep 1, 1993·The American Journal of Physiology·G R Dubyak, C el-Moatassim
Sep 1, 1993·The Journal of Cell Biology·N Panté, U Aebi
Jan 17, 1996·Biochemical and Biophysical Research Communications·S K KongC Y Lee
Jul 1, 1996·The Journal of Physiology·H Shirakawa, S Miyazaki
Mar 1, 1996·The Journal of Physiology·M MoriY Okada
Apr 1, 1996·The American Journal of Physiology·H YamadaK Kurokawa

❮ Previous
Next ❯

Citations

May 8, 2001·Clinical and Experimental Pharmacology & Physiology·E W Inscho
Jan 31, 2016·Critical Care Medicine·Hailin ZhaoDaqing Ma
Apr 8, 2004·American Journal of Physiology. Renal Physiology·Shen-Ling XiaCharles S Wingo
Sep 30, 1999·Microscopy Research and Technique·S A Stricker, M Whitaker
Feb 24, 2001·American Journal of Physiology. Renal Physiology·R NasrallahR L Hébert
May 16, 2001·American Journal of Physiology. Renal Physiology·E W Inscho
May 16, 2001·American Journal of Physiology. Renal Physiology·E M Schwiebert, B K Kishore

❮ Previous
Next ❯

Related Concepts

Related Feeds

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.