Nuclear and microtubule reorganization in nuclear-transferred bovine embryos

Molecular Reproduction and Development
Mi-Ra ShinNam-Hyung Kim

Abstract

We studied the nuclear and microtubule dynamics in nonactivated and pre-activated chromatin-removed oocytes following transfer of nuclei from bovine fibroblast cells. Immediately after fusion between membranes of oocytes and fibroblasts, a microtubule aster containing a gamma-tubulin spot was seen near the transferred nucleus in most oocytes regardless of activation conditions. Most fibroblast nuclei transferred into nonactivated oocytes underwent premature chromosome condensation (PCC) and divided into two masses of chromosomes. In contrast, fibroblast nuclei in pre-activated oocytes rarely underwent PCC, but formed a swelled pronuclear-like structure. Under nonactivation condition, microtubular spindles surrounded condensed chromosomes during the division of two nuclear structures. Gamma-tubulins were detected in the vicinity of condensed chromosomes, suggesting transient spindle formation. Two pronuclear-like structures near the microtubular aster containing gamma-tubulin spot(s) later formed a syngamy-like nuclear structure. While 20% of reconstructed oocytes under nonactivated conditions developed to morulae and blastocysts, only 4% of reconstructed oocytes under pre-activated conditions developed to morulae and blastocyst...Continue Reading

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Citations

Jan 22, 2004·Cloning and Stem Cells·Mayuko KuromeHiroshi Nagashima
Jul 15, 2009·Cloning and Stem Cells·Olga ØstrupPoul Maddox-Hyttel
Sep 10, 2008·Yi chuan = Hereditas·Wei-Hua DuDong Wang
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Jul 22, 2004·IEEE Engineering in Medicine and Biology Magazine : the Quarterly Magazine of the Engineering in Medicine & Biology Society·Nam-Hyung KimYong Man Han
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Jul 6, 2007·Developmental Biology·Sebastian T BalbachMichele Boiani
Mar 25, 2004·Molecular Reproduction and Development·Sang-Hyun ParkNam-Hyung Kim
Jul 12, 2018·Cell Biology International·Hai-Jun Liu, Ru-Ming Liu
Jan 30, 2007·Reproduction, Fertility, and Development·Gábor VajtaZoltán Macháty
Oct 12, 2010·Molecular Reproduction and Development·Kristin M Whitworth, Randall S Prather
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