Nuclear and mitochondrial genes for inferring Trichuris phylogeny

Parasitology Research
Rocío CallejónS A Nadler

Abstract

Nucleotide sequences of the triose phosphate isomerase (TPI) gene (624 bp) and mitochondrial cytochrome b (cob) gene (520 bp) were obtained by PCR and evaluated for utility in inferring the phylogenetic relationships among Trichuris species. Published sequences of one other nuclear gene (18S or SSU rRNA, 1816-1846 bp) and one additional mitochondrial (mtDNA) gene (cytochrome oxidase 1, cox1, 342 bp) were also analyzed. Maximum likelihood and Bayesian inference methods were used to infer phylogenies for each gene separately but also for the combined mitochondrial data (two genes), the combined nuclear data (two genes), and the total evidence (four gene) dataset. Few Trichuris clades were uniformly resolved across separate analyses of individual genes. For the mtDNA, the cob gene trees had greater phylogenetic resolution and tended to have higher support values than the cox1 analyses. For nuclear genes, the SSU gene trees had slightly greater resolution and support values than the TPI analyses, but TPI was the only gene with reliable support for the deepest nodes in the tree. Combined analyses of genes yielded strongly supported clades in most cases, with the exception of the relationship among Trichuris clades 1, 2, and 3, which...Continue Reading

References

Feb 1, 1992·Journal of Molecular Evolution·P J LockhartA W Larkum
Jul 6, 2000·Baillière's Best Practice & Research. Clinical Rheumatology·P J Maddison
Jun 26, 2003·Nucleic Acids Research·Rasmus Wernersson, Anders Gorm Pedersen
Aug 13, 2003·Bioinformatics·Ari Löytynoja, Michel C Milinkovitch
Aug 13, 2003·Bioinformatics·Fredrik Ronquist, John P Huelsenbeck
Dec 14, 2004·Gut·R W SummersJ V Weinstock
Sep 28, 2006·Parasitology Research·Cristina CutillasDiego Guevara
Jan 9, 2007·Parasitology Research·Aditya Reddy, Bernard Fried
Feb 23, 2007·International Journal for Parasitology·David E ElliottJoel V Weinstock
Mar 10, 2007·Systematic Biology·Ashleigh B SmytheSteven A Nadler
Sep 12, 2007·Bioinformatics·M A LarkinD G Higgins
Dec 1, 2007·WormBook : the Online Review of C. Elegans Biology·David M Eisenmann
May 27, 2009·Acta Tropica·C CutillasD C Guevara
Nov 21, 2009·The Journal of Parasitology·Gerardo Pérez-Ponce de León, Steven A Nadler
Aug 8, 2013·Veterinary Parasitology·Lisa GuardoneAlexander Mathis

❮ Previous
Next ❯

Citations

Jan 24, 2016·Parasites & Vectors·Mohamed B F HawashPeter Nejsum
Jan 27, 2021·Veterinary Sciences·Kegan Romelle Jones
Nov 19, 2020·BMC Veterinary Research·Julia RiveroRocío Callejón

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.