Nuclear and nucleolar localization of 18-kDa fibroblast growth factor-2 is controlled by C-terminal signals.

The Journal of Biological Chemistry
Zhi ShengWilliam J Chirico

Abstract

Members of high (22-, 22.5-, 24-, and 34-kDa) and low (18-kDa) molecular mass forms of fibroblast growth factor-2 (FGF-2) regulate cell proliferation, differentiation, and migration. FGF-2s have been previously shown to accumulate in the nucleus and nucleolus. Although high molecular weight forms of FGF-2 contain at least one nuclear localization signal (NLS) in their N-terminal extension, the 18-kDa FGF-2 does not contain a standard NLS. To determine signals controlling the nuclear and subnuclear localization of the 18-kDa FGF-2, its full-length cDNA was fused to that of green fluorescent protein (GFP). The fusion protein was primarily localized to the nucleus of COS-7 and HeLa cells and accumulated in the nucleolus. The subcellular distribution was confirmed using wild type FGF-2 and FGF-2 tagged with a FLAG epitope. A 17-amino acid sequence containing two groups of basic amino acid residues separated by eight amino acid residues directed GFP and a GFP dimer into the nucleus. We systematically mutated the basic amino acid residues in this nonclassical NLS and determined the effect on nuclear and nucleolar accumulation of 18-kDa FGF-2. Lys(119) and Arg(129) are the key amino acid residues in both nuclear and nucleolar localiza...Continue Reading

References

Nov 16, 1992·Biochemical and Biophysical Research Communications·X ZhanT Maciag
Jan 1, 1991·Growth Factors·R Z FlorkiewiczA M Gonzalez
Jan 1, 1991·Methods in Enzymology·S A ThompsonJ A Abraham
May 1, 1991·Journal of Cellular Physiology·N QuartoD B Rifkin
Jul 1, 1990·Journal of Cellular Physiology·M RenkoD B Rifkin
Nov 1, 1990·Journal of Cellular Physiology·S Tessler, G Neufeld
Oct 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·G BoucheF Amalric
Oct 24, 1998·The Journal of Cell Biology·T Pederson
Dec 10, 1998·Nucleic Acids Research·K HofmannA Bairoch
Jan 19, 1999·Methods in Cell Biology·D W PistonS M Knobel
May 4, 1999·The Journal of Cell Biology·M Keresztes, J Boonstra
Jun 8, 2000·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·D A JansM H Lam
Oct 6, 2000·Endocrine-related Cancer·C J PowersA Wellstein
Mar 22, 2001·EMBO Reports·M CokolB Rost
Mar 29, 2001·Genome Biology·D M Ornitz, N Itoh
May 23, 2001·The Biochemical Journal·M Dundr, T Misteli
Jun 21, 2001·Biochemical and Biophysical Research Communications·Y D SohnY Jang
Jan 26, 2002·Molecular and Cellular Biology·Frédéric CatezJean-Jacques Diaz
Jan 17, 2003·Molecular and Cellular Biology·Odile FilholClaude Cochet
Feb 25, 2003·Protein Expression and Purification·Zhi ShengWilliam J Chirico
Apr 16, 2003·The Journal of Biological Chemistry·Marcos R M FontesBostjan Kobe
Nov 18, 2003·Cellular and Molecular Life Sciences : CMLS·A FolettiF Beermann

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Citations

Oct 14, 2008·Cellular and Molecular Life Sciences : CMLS·K ChlebovaP Krejci
Jan 30, 2013·Plant Molecular Biology·Raghavendra P Savada, Peta C Bonham-Smith
May 18, 2005·Biochemical and Biophysical Research Communications·Yifat Rosenbaum-DekelBen-Zion Levi
Jan 17, 2007·Nature Reviews. Microbiology·Julian A Hiscox
Nov 21, 2007·DNA and Cell Biology·Michal K StachowiakEwa K Stachowiak
Oct 10, 2007·Molecular and Cellular Biology·Lingjun MengRobert Y L Tsai
Oct 18, 2005·Molecular and Cellular Biology·Zhi ShengWilliam J Chirico
Aug 30, 2013·Virology Journal·Saijo ThomasOttmar Herchenröder
Dec 13, 2006·Journal of Cell Science·Lingjun MengRobert Y L Tsai
Dec 18, 2008·Biological Chemistry·Alexander-Francisco BrunsPeter Claus
Mar 7, 2006·FEBS Letters·Petr DvorakAles Hampl
Dec 17, 2014·Journal of Cellular Physiology·Michal K StachowiakE K Stachowiak
Jul 28, 2009·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Ji WangLi Wang
Jan 30, 2007·Acta histochemica·Eric J Arnoys, John L Wang
Sep 6, 2008·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Siyun LiaoJo El J Schultz
Mar 5, 2008·Molecular Reproduction and Development·Shobhit SaxenaSatish Totey
Feb 21, 2009·EMBO Reports·Edward Emmott, Julian A Hiscox
Feb 15, 2005·Acta histochemica·Flavio LejbkowiczAndrew P Levy
Jun 26, 2007·Analytical Biochemistry·Nicole Maria SeibelWolfgang Hampe
Jan 2, 2015·Cellular and Molecular Life Sciences : CMLS·Benjamin FörthmannPeter Claus
Nov 26, 2009·Biochemical and Biophysical Research Communications·Heyu ZhangDalong Ma
Sep 19, 2018·Molecular and Cellular Biology·Daniel J SalamangoReuben S Harris
Apr 15, 2006·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Vigdis SørensenAntoni Wiedłocha
May 2, 2007·Journal of Cellular Biochemistry·Jeffrey KosmanJames A Bassuk
May 3, 2019·Nucleic Acids Research·Daria GudkovaWinship Herr
Oct 7, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Azusa Inoue, Fugaku Aoki
Aug 29, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jeremy R HershfieldAryan Namboodiri
Mar 4, 2008·The FEBS Journal·Simona TavernaMaria Letizia Vittorelli
Jul 10, 2007·The Journal of Biological Chemistry·Trine NilsenAntoni Wiedłocha
Sep 12, 2015·Immunogenetics·Daryl A TaketaAnthony W De Tomaso
Aug 29, 2013·Dalton Transactions : an International Journal of Inorganic Chemistry·Anna LeonidovaGilles Gasser
May 10, 2020·Nucleic Acids Research·Seoung Min BongByung Il Lee
Sep 17, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Philip D Da FornoGerald S Saldanha

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