Nuclear, chloroplast, and mitochondrial data of a US cannabis DNA database

International Journal of Legal Medicine
Rachel HoustonDavid Gangitano

Abstract

As Cannabis sativa (marijuana) is a controlled substance in many parts of the world, the ability to track biogeographical origin of cannabis could provide law enforcement with investigative leads regarding its trade and distribution. Population substructure and inbreeding may cause cannabis plants to become more genetically related. This genetic relatedness can be helpful for intelligence purposes. Analysis of autosomal, chloroplast, and mitochondrial DNA allows for not only prediction of biogeographical origin of a plant but also discrimination between individual plants. A previously validated, 13-autosomal STR multiplex was used to genotype 510 samples. Samples were analyzed from four different sites: 21 seizures at the US-Mexico border, Northeastern Brazil, hemp seeds purchased in the US, and the Araucania area of Chile. In addition, a previously reported multi-loci system was modified and optimized to genotype five chloroplast and two mitochondrial markers. For this purpose, two methods were designed: a homopolymeric STR pentaplex and a SNP triplex with one chloroplast (Cscp001) marker shared by both methods for quality control. For successful mitochondrial and chloroplast typing, a novel real-time PCR quantitation method w...Continue Reading

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Citations

Aug 25, 2019·International Journal of Legal Medicine·Madeline G RomanRachel Houston
Jun 26, 2020·International Journal of Legal Medicine·Lucas de Oliveira Pereira RibeiroClarice Sampaio Alho
Jan 2, 2021·Forensic Science International : Synergy·Nicole S Jones, Jeffrey H Comparin
Aug 28, 2020·Forensic Science International : Synergy·Mauro Sander FettFlávio Anastácio de Oliveira Camargo
Nov 1, 2020·Forensic Science International : Synergy·Jaime SolanoDavid Gangitano
Nov 30, 2018·Analytical Chemistry·Bruce R McCordGeorge Duncan

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