Nuclear cytometry and chromatin organization

Cytometry. Part a : the Journal of the International Society for Analytical Cytology
Paul J SmithRachel J Errington

Abstract

The nuclear-targeting chemical probe, for the detection and quantification of DNA within cells, has been a mainstay of cytometry-from the colorimetric Feulgen stain to smart fluorescent agents with tuned functionality. The level of nuclear structure and function at which the probe aims to readout, or indeed at which a DNA-targeted drug acts, is shadowed by a wide range of detection modalities and analytical methods. These methods are invariably limited in terms of the resolution attainable versus the volume occupied by targeted chromatin structures. The scalar challenge arises from the need to understand the extent and different levels of compaction of genomic DNA and how such structures can be re-modeled, reported, or even perturbed by both probes and drugs. Nuclear cytometry can report on the complex levels of chromatin order, disorder, disassembly, and even active disruption by probes and drugs. Nuclear probes can report defining features of clinical and therapeutic interest as in NETosis and other cell death processes. New cytometric approaches continue to bridge the scalar challenges of analyzing chromatin organization. Advances in super-resolution microscopy address the resolution and depth of analysis issues in cellular ...Continue Reading

References

Dec 1, 1977·Canadian Journal of Genetics and Cytology. Journal Canadien De Génétique Et De Cytologie·S A Latt
Jan 1, 1979·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Z DarzynkiewiczM R Melamed
Jan 1, 1978·Methods in Cell Biology·E J Gabbay, W D Wilson
Dec 1, 1979·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·H A CrissmanR J Kissane
Dec 1, 1979·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A Krishan, R Ganapathi
Jul 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J BartkowiakZ Darzynkiewicz
Jan 25, 1988·Biochimica Et Biophysica Acta·D H Bell
Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J Kapuscinski, Z Darzynkiewicz
Jul 1, 1986·European Journal of Cancer & Clinical Oncology·B S AnderssonK B McCredie
Dec 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·S A Latt
Jan 1, 1970·Chromosoma·T CasperssonE J Modest
May 1, 1974·Chemico-biological Interactions·P F Lurquin
Sep 1, 1980·Cytometry·Z DarzynkiewiczM R Melamed
Jan 1, 1983·Cytogenetics and Cell Genetics·T Matsubara, Y Nakagome
Mar 15, 1982·Biochemical Pharmacology·H Waldes, M S Center
Apr 30, 1980·Biochimica Et Biophysica Acta·F ZuninoR A Gambetta
Dec 5, 1980·Science·D P EvensonM R Melamed
Apr 1, 1996·The Journal of Experimental Medicine·M A GoodellR C Mulligan
Oct 19, 2000·Experimental Cell Research·H D HalickaZ Darzynkiewicz
May 4, 2001·Micron : the International Research and Review Journal for Microscopy·J Dobrucki, Z Darzynkiewicz
Jun 8, 2002·Current Pharmaceutical Design·L H Patterson, G I Murray
Mar 6, 2004·Science·Volker BrinkmannArturo Zychlinsky
Apr 2, 2004·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Xuan HuangZbigniew Darzynkiewicz
Apr 20, 2004·Mathematical Biosciences·Neil D EvansMichael J Chappell
Jun 29, 2004·Biochemical and Biophysical Research Communications·G BreuzardJ-M Millot
Dec 22, 2004·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Azra RabbaniJuan Ausió
Feb 22, 2005·Biochemical and Biophysical Research Communications·G BreuzardJ-M Millot
Aug 6, 2005·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Robert M MartinM Cristina Cardoso
Oct 1, 2005·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·M Titford
Feb 4, 2006·Applied Spectroscopy·Christian MatthäusMax Diem
Mar 22, 2006·Cellular Microbiology·Constantin F UrbanArturo Zychlinsky
May 23, 2006·Current Opinion in Structural Biology·Philip J J Robinson, Daniela Rhodes
Sep 14, 2006·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Kerenza L NjohPaul J Smith

❮ Previous
Next ❯

Citations

Nov 14, 2018·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·H Dorota HalickaZbigniew Darzynkiewicz
Nov 26, 2019·Current Protocols in Cytometry·Zbigniew DarzynkiewiczJiangwei Li

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Nature Biotechnology
Joshua C Vaughan, Xiaowei Zhuang
Médecine sciences : M/S
Ignacio IzeddinMaxime Dahan
Organic & Biomolecular Chemistry
Peyton Shieh, Carolyn R Bertozzi
© 2022 Meta ULC. All rights reserved