Nuclear degeneration in the developing lens and its regulation by TNFalpha

Experimental Eye Research
M A Wride, E J Sanders

Abstract

DNA fragmentation in lens fibre cell nuclei undergoing programmed degeneration was identified by terminal deoxynucleotidyl transferase (TdT)-mediated biotin-dUTP nick end labelling (TUNEL). Lens epithelial cells in culture were induced to differentiate into lens fibre-like clumps of cells (lentoids) by insulin and it was shown that the TUNEL method was also an effective means of labelling degenerating nuclei in lentoid cells in lens epithelial cell cultures. Using immuno-fluorescence and confocal microscopy, it was shown that TNFalpha and TNF receptor (TNFR1, and TNFR2) immunoreactivity was present in sections of chick embryo lenses. TNFalpha immunoreactivity was associated with the lens epithelium and lens fibres. TNFR1 immunoreactivity was present in lens epithelial cells, cortical lens fibres, and lens fibre cell nuclei, while TNFR2 immunoreactivity had a similar distribution to that of TNFR1, but was not associated with nuclei. Similar patterns of TNFalpha, TNFR1, and TNFR2 immunoreactivity were observed in lens epithelial cell cultures. When added to lens epithelial cell cultures, TNFalpha, at concentrations of 50 to 100 ng ml-1, and agonistic antibodies to both TNFR1 and TNFR2 significantly (P<0.05) enhanced the number of...Continue Reading

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Citations

Mar 16, 2011·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Michael A Wride
Nov 26, 2009·Experimental Eye Research·Noemi LoisColin McCaig
Mar 12, 2009·Differentiation; Research in Biological Diversity·Christina E LorénLena Gunhaga
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Apr 20, 2006·Birth Defects Research. Part C, Embryo Today : Reviews·Michael A WrideJeremy A Guggenheim
Sep 13, 2003·Differentiation; Research in Biological Diversity·Esmond J Sanders, Eve Parker
Mar 7, 2002·Experimental Eye Research·Steven Bassnett
May 9, 2006·Developmental Biology·Gregory F Weber, A Sue Menko
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Oct 29, 2003·The Journal of Experimental Biology·Steven Bassnett, Richard McNulty
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Dec 12, 2003·Experimental Eye Research·P ValverdeA Taylor

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