Nuclear diacylglycerol kinase-theta is activated in response to alpha-thrombin

The Journal of Biological Chemistry
L BregoliDaniel M Raben

Abstract

Currently, there is substantial evidence that nuclear lipid metabolism plays a critical role in a number of signal transduction cascades. Previous work from our laboratory showed that stimulation of quiescent fibroblasts with alpha-thrombin leads to the production of two lipid second messengers in the nucleus: an increase in nuclear diacylglycerol mass and an activation of phospholipase D, which catalyzes the hydrolysis of phosphatidylcholine to generate phosphatidic acid. Diacylglycerol kinase (DGK) catalyzes the conversion of diacylglycerol to phosphatidic acid, making it an attractive candidate for a signal transduction component. There is substantial evidence that this activity is indeed regulated in a number of signaling cascades (reviewed by van Blitterswijk, W. J., and Houssa, B. (1999) Chem. Phys. Lipids 98, 95-108). In this report, we show that the addition of alpha-thrombin to quiescent IIC9 fibroblasts results in an increase in nuclear DGK activity. The examination of nuclei isolated from quiescent IIC9 cells indicates that DGK-theta and DGK-delta are both present. We took advantage of the previous observations that phosphatidylserine inhibits DGK-delta (reviewed by Sakane, F., Imai, S., Kai, M., Wada, I., and Kanoh,...Continue Reading

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Citations

May 29, 2004·Biochimica Et Biophysica Acta·Alrik P LosWim J van Blitterswijk
Jun 12, 2003·Experimental Cell Research·Giovanna TabelliniAlberto M Martelli
Jul 19, 2002·Advances in Enzyme Regulation·Lisa BregoliDaniel M Raben
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Sep 6, 2011·Annual Review of Physiology·Natasha C Lucki, Marion B Sewer
Nov 17, 2009·Advances in Enzyme Regulation·Becky Tu-Sekine, Daniel M Raben
Sep 30, 2016·Frontiers in Cell and Developmental Biology·Becky Tu-SekineDaniel M Raben
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