Nuclear distributions of NUP62 and NUP214 suggest architectural diversity and spatial patterning among nuclear pore complexes.

PloS One
Yayoi KinoshitaD Stave Kohtz

Abstract

The shape of nuclei in many adherent cultured cells approximates an oblate ellipsoid, with contralateral flattened surfaces facing the culture plate or the medium. Observations of cultured cell nuclei from orthogonal perspectives revealed that nucleoporin p62 (NUP62) and nucleoporin 214 (NUP214) are differentially distributed between nuclear pore complexes on the flattened surfaces and peripheral rim of the nucleus. High resolution stimulated emission depletion (STED) immunofluorescence microscopy resolved individual NPCs, and suggested both heterogeneity and microheterogeneity in NUP62 and NUP214 immunolabeling among in NPC populations. Similar to nuclear domains and interphase chromosome territories, architectural diversity and spatial patterning of NPCs may be an intrinsic property of the nucleus that is linked to the functions and organization of underlying chromatin.

References

Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·L I Davis, G Blobel
Feb 15, 1994·Proceedings of the National Academy of Sciences of the United States of America·D KraemerA Radu
Jan 1, 1995·Journal of Cell Science. Supplement·N Panté, U Aebi
Apr 1, 1996·Critical Reviews in Biochemistry and Molecular Biology·N Panté, U Aebi
Dec 8, 1998·Molecular Biology of the Cell·N BelgarehV Doye
Aug 19, 2000·In Vitro Cellular & Developmental Biology. Animal·D M ProvencherA M Mes-Masson
Mar 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·X WuJ M van Deursen
Jul 13, 2001·The Journal of Cell Biology·S K Lyman, L Gerace
May 22, 2002·International Review of Cytology·Susanne M Steggerda, Bryce M Paschal
Jul 9, 2002·Molecular and Cellular Biology·Isabelle C BraunElisa Izaurralde
Aug 17, 2002·Canadian Journal of Physiology and Pharmacology·P JacquetC Desaintes
Dec 20, 2002·Journal of Structural Biology·Birthe FahrenkrogUeli Aebi
Dec 3, 2003·Molecular & Cellular Proteomics : MCP·Heidi J RayalaSusan R Wente
Feb 11, 2005·Molecular Biology of the Cell·Lisa A Hawryluk-GaraRichard W Wozniak
Apr 20, 2005·Current Opinion in Structural Biology·Thomas U Schwartz
Jul 28, 2005·Journal of Molecular Biology·Sara M PaulilloBirthe Fahrenkrog
Apr 25, 2006·Current Opinion in Cell Biology·Roderick Y H Lim, Birthe Fahrenkrog
May 6, 2006·The Journal of Biological Chemistry·Rafael BernadMaarten Fornerod
May 10, 2006·Current Opinion in Cell Biology·Charles N Cole, John J Scarcelli
May 17, 2006·The Journal of Cell Biology·Fabrizia StavruDirk Görlich
May 17, 2006·The Journal of Cell Biology·Fabrizia StavruDirk Görlich
Sep 12, 2006·Journal of Molecular Biology·Sara M PaulilloBirthe Fahrenkrog
Oct 13, 2006·Journal of Cell Science·Nikos XylourgidisChristos Samakovlis
Nov 1, 2006·Journal of Cell Science·Kazuhiro MaeshimaNaoko Imamoto
May 15, 2007·The Journal of Biological Chemistry·Yumei LengDonald Kufe
Jun 15, 2007·Trends in Genetics : TIG·Sara Ahmed, Jason H Brickner
Aug 8, 2007·Developmental Cell·Bernike Kalverda, Maarten Fornerod
Aug 8, 2007·The Journal of Cell Biology·Nafiseh SabriChristos Samakovlis
Sep 19, 2007·The Journal of Cell Biology·Laura J Terry, Susan R Wente
Nov 30, 2007·Nature·Frank AlberMichael P Rout

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Citations

Jan 23, 2014·Mechanisms of Ageing and Development·Boris Fichtman, Amnon Harel
Oct 24, 2012·Nature Reviews. Molecular Cell Biology·Marcela Raices, Maximiliano A D'Angelo
Oct 29, 2014·Proceedings of the National Academy of Sciences of the United States of America·Yayoi KinoshitaD Stave Kohtz
Nov 8, 2015·Trends in Cell Biology·Andrew M SydorVito Mennella
Oct 11, 2013·Cell Cycle·Chieko HashizumeRichard W Wong
Feb 14, 2019·Human Genetics·Hanan E ShamseldinFowzan S Alkuraya
May 30, 2020·Viruses·Luis Adrián De Jesús-GonzálezRosa María Del Ángel
Jul 28, 2016·BMC Systems Biology·Teresa Vaz MartinsRichard J Morris
Jan 24, 2018·Journal of Cell Science·Alicia A CutlerGrace K Pavlath
Feb 11, 2021·Trends in Biochemical Sciences·Javier Fernandez-Martinez, Michael P Rout
Jul 12, 2017·Bioscience Reports·Sviatlana Shashkova, Mark C Leake
May 4, 2013·Journal of Cell Science·Shige H YoshimuraKunio Takeyasu

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Methods Mentioned

BETA
nucleotide exchange
transfection
confocal microscopy
light diffraction

Software Mentioned

Systat
TableCurve
Image J
AutoQuant

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