Nuclear dynamics of parthenogenesis of human oocytes: effect of oocyte aging in vitro

Gynecologic and Obstetric Investigation
Kazuo SengokuMutsuo Ishikawa

Abstract

We investigated in detail the nuclear kinetics of oocyte activation of aged human oocytes following combined activation treatment with calcium ionophore and puromycin. Two types of oocytes were used: (a) 1-day-old oocytes after 20-24 h retrieval, and (b) 2-day-old oocytes after 44-50 h retrieval. A total of 185 unfertilized aged oocytes, 91 1-day-old and 94 2-day-old oocytes, were fixed at 1, 2, 4, 6 and 8 h after activation treatment and then metaphase II (MII), anaphase or telophase II (A/T II) or pronuclear stage were recorded. We demonstrated that a combined calcium ionophore and puromycin treatment induced a high activation rate in both 1-day-old (95.6%) and 2-day-old oocytes (95.2%). Our results also demonstrated that the nuclear progression was faster in 2-day-old oocytes than in 1-day-old oocytes, although nuclear progression in parthenogenetically activated human oocytes requires the longer time periods compared with ICSI fertilization. It is concluded that combined treatment of the calcium ionophore and puromycin allows a high rate of parthenogenetic activation and the nuclear kinetics of parthenogenetically activated human oocytes appears to be more rapid in in vitro aging oocytes.

References

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Citations

Jun 13, 2008·Stem Cell Reviews·Tiziana A L BreviniFulvio Gandolfi
Jan 26, 2016·Fertility and Sterility·María-José EscribáAntonio Pellicer
Jan 10, 2008·Cell Proliferation·T A L Brevini, F Gandolfi
Oct 30, 2016·Journal of Cellular Physiology·Meenakshi TiwariShail K Chaube
Jan 24, 2020·Reproduction in Domestic Animals = Zuchthygiene·Fumei ChenMing Zhang
Jun 6, 2013·Experimental and Therapeutic Medicine·Bao-Sheng Han, Jun-Ling Gao
Aug 21, 2007·Fertility and Sterility·Ester Polak de FriedJosé Cibelli

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