PMID: 7524027Sep 11, 1994Paper

Nuclear-encoded mitochondrial tRNAs of Trypanosoma brucei have a modified cytidine in the anticodon loop

Nucleic Acids Research
A SchneiderN Agabian

Abstract

The mitochondrial genome of Trypanosoma brucei does not appear to encode any tRNA genes. Isolated organellar tRNAs hybridize to nuclear DNA, suggesting that they are synthesized in the nucleus and subsequently imported into the mitochondrion. Most imported tRNAs have cytosolic counterparts, showing identical mobility on two-dimensional polyacrylamide gels. We have compared three nuclear-encoded mitochondrial tRNAs (tRNA(Lys), tRNA(Leu), tRNA(Tyr)) with their cytosolic isoforms by direct enzymatic sequence analysis. Our findings indicate that the primary sequences of the mitochondrial and the corresponding cytosolic tRNAs are identical. However, we have identified a mitochondrion-specific nucleotide modification of each tRNA which is localized to a conserved cytidine residue at the penultimate position 5' of the anticodon. The modification present in mature mitochondrial tRNA(Tyr) was not found in a mutant tRNA(Tyr) defective in splicing in either cytosolic or mitochondrial fractions. The mutant tRNA(Tyr) has been expressed in transformed cells and its import into mitochondria has been demonstrated, suggesting that the modified cytidine residue is not required for import and therefore may be involved in adapting imported tRNAs t...Continue Reading

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Citations

Jan 1, 1996·Biochimie·I A Tarassov, R P Martin
Dec 21, 2000·Trends in Cell Biology·A Schneider, L Maréchal-Drouard
Apr 24, 2009·The Journal of Biological Chemistry·Fabien CharrièreAndré Schneider
Aug 6, 1999·Molecular Biology of the Cell·C E NabholzA Schneider
Apr 6, 2004·Molecular Biology of the Cell·Anne Crausaz EsseivaAndré Schneider
Jan 13, 2004·Nucleic Acids Research·Paul F Agris
May 9, 2002·Molecular and Cellular Biology·Timothy H P TanAndré Schneider
May 23, 2007·Molekuliarnaia biologiia·P A KamenskiĭI A Tarasov
Jan 17, 2002·Proceedings of the National Academy of Sciences of the United States of America·Timothy H P TanAndre Schneider
Oct 31, 2009·The Journal of Biological Chemistry·Ellen Inga BruskeAndré Schneider
May 17, 2005·FEBS Letters·Takehiro YasukawaKimitsuna Watanabe
Mar 4, 2015·International Journal of Molecular Sciences·Thalia Salinas-GiegéPhilippe Giegé
Jan 21, 2014·Biochimie·Jonathan L HuotLaurence Maréchal-Drouard
Oct 26, 2010·Molecular Microbiology·Marina CristoderoAndré Schneider
Jul 20, 1999·The Journal of Biological Chemistry·A J LeBlancS L Hajduk
Sep 20, 2000·The Journal of Biological Chemistry·S T KapushocL Simpson
Dec 1, 2005·The Journal of Biological Chemistry·Naoki ShigiKimitsuna Watanabe

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