Nuclear exclusion of p33ING1b tumor suppressor protein: explored in HCC cells using a new highly specific antibody

Hybridoma
Berna SayanRengul Cetin-Atalay

Abstract

Mouse monoclonal antibodies (MAb) were generated against p33ING1b tumor suppressor protein. 15B9 MAb was highly specific in recognizing a single protein band of approximately 33 kDa endogenous p33ING1b protein from HCC cell lines and normal liver tissue by Western blot analysis and by immunoprecipitation. Although p33ING1b mutations are rarely observed in cancer, differential subcellular distribution and nuclear exclusion of p33ING1b were reported in different cancer types. Therefore we analyzed the expression and subcellular localization of p33ING1b in HCC cell lines using 15B9 MAb. So far, p33ING1b mutations or differential subcellular localization are not reported in HCC. In this study, by indirect immunofluorescence using MAb 15B9, we demonstrate that nuclear localization of p33ING1b was highly correlated with well-differentiated HCC cell lines whereas poorly differentiated HCC cells have nuclear exclusion of the protein. Moreover no association was observed between differential subcellular localization of p33ING1b and p53 mutation status of HCC cell lines. Hence our newly produced MAb 15B9 can be used for studying cellular activities of p33ING1b under normal and cancerous conditions.

References

Nov 15, 2000·British Journal of Cancer·K J CheungG Li
May 17, 2002·The Journal of Biological Chemistry·Diego VieyraKarl Riabowol
Nov 26, 2002·Trends in Cell Biology·Xiaolan FengKarlT Riabowol
Jul 2, 2003·Journal of Clinical Pathology·G S NoumanB Angus
Oct 24, 2003·FEBS Letters·Fan Cheung TsangRandy Y C Poon
Sep 10, 2004·Molecular Biology and Evolution·Gordon H Y HeKarl Riabowol
Mar 4, 2005·The International Journal of Biochemistry & Cell Biology·Wei GongKarl Riabowol
Apr 1, 2005·World Journal of Gastroenterology : WJG·Zhi ZhuMing-Hua Zhu
Mar 7, 2007·Nature Reviews. Genetics·Manel Esteller
Oct 24, 2007·Trends in Biochemical Sciences·Mohamed A Soliman, Karl Riabowol
Jun 10, 2008·Current Cancer Drug Targets·Mehmet GunduzHitoshi Nagatsuka

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Citations

Jul 16, 2010·Investigational New Drugs·Francesca BuontempoMaria Luca Neri

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