Nuclear factor 1 and octamer transcription factor 1 binding preset the chromatin structure of the mouse mammary tumor virus promoter for hormone induction.

The Journal of Biological Chemistry
Sergey BelikovÖrjan Wrange

Abstract

When the mouse mammary tumor virus (MMTV) is integrated into the genome of a mammalian cell, its long terminal repeat (LTR) harbors six specifically positioned nucleosomes. Transcription from the MMTV promoter is regulated by the glucocorticoid hormone via the glucocorticoid receptor (GR). The mechanism of the apparently constitutive nucleosome arrangement has remained unclear. Previous in vitro reconstitution of nucleosome(s) on small segments of the MMTV LTR suggested that the DNA sequence was decisive for the nucleosome arrangement. However, microinjection of MMTV LTR DNA in Xenopus oocytes rendered randomly distributed nucleosomes. This indicated that oocytes lack factor(s) that induces nucleosome positioning at the MMTV LTR in other cells. Here we demonstrate that specific and concomitant binding of nuclear factor 1 (NF1) and octamer factor 1 (Oct1) to their cognate sites within the MMTV promoter induce a partial nucleosome positioning that is an intermediary state between the randomly organized inactive promoter and the hormone and GR-activated promoter containing distinctly positioned nucleosomes. Oct1 and NF1 reciprocally facilitate each other's binding to the MMTV LTR in vivo. The NF1 and Oct1 binding also facilitate h...Continue Reading

References

May 1, 1992·The Biochemical Journal·W H Günzburg, B Salmons
Feb 1, 1992·Molecular and Cellular Biology·C S HinkleyM Perry
Jan 1, 1991·Progress in Nucleic Acid Research and Molecular Biology·R T Simpson
Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·T E Shrader, D M Crothers
Dec 20, 1985·Journal of Molecular Biology·H R Drew, A A Travers
Oct 23, 1970·Science·T J LindellW J Rutter
Jun 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·I L CartwrightS C Elgin
Mar 1, 1994·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·L L WallrathS C Elgin
Dec 1, 1996·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·M Beato
Sep 15, 1997·Nucleic Acids Research·M Beato, K Eisfeld
Jun 20, 1998·Molecular and Cellular Biology·G G PréfontaineR J Haché
Mar 4, 2000·The EMBO Journal·S BelikovO Wrange
Oct 29, 2000·Progress in Nucleic Acid Research and Molecular Biology·G L Hager
Feb 24, 2001·Journal of Molecular Biology·K Phillips, B Luisi
Jul 20, 2002·Experimental Cell Research·Xiang Hui FuChih-Chuan Liang
Mar 7, 2003·Molecular Cell·Shilpa Kadam, Beverly M Emerson
Aug 9, 2003·Science·Shiv I S Grewal, Danesh Moazed
Dec 4, 2003·Molecular and Cellular Biology·Cindy J BachurskiDihua Hong

❮ Previous
Next ❯

Citations

Jun 15, 2010·Nucleic Acids Research·Richard C Stolz, Thomas C Bishop
Jan 11, 2007·Molecular and Cellular Biology·Sergey BelikovOrjan Wrange
Aug 16, 2011·Progress in Biophysics and Molecular Biology·Denis Michel
May 20, 2008·Journal of Molecular Biology·Michael G PoirierJonathan Widom
Mar 8, 2005·Experimental Cell Research·Per-Henrik HolmqvistOrjan Wrange
Apr 25, 2009·Journal of Molecular Biology·Zhigang KangEsther M Sternberg
Jan 12, 2017·International Journal of Cancer. Journal International Du Cancer·Mitchell FaneMichael Piper
Dec 4, 2008·Stress : the International Journal on the Biology of Stress·Simon C Biddie, Gordon L Hager

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.