Nuclear factor erythroid 2-related factor 2 (Nrf2) regulates airway epithelial barrier integrity

Allergology International : Official Journal of the Japanese Society of Allergology
Yoshitaka ShintaniShu Hashimoto

Abstract

Inhaled corticosteroids enhance airway epithelial barrier integrity. However, the mechanism by which they accomplish this is unclear. Therefore, we investigated steroid-inducible genes and signaling pathways that were involved in enhancing airway epithelial barrier integrity. A human bronchial epithelial cell line (16HBE cells) was cultured with 10(-6) M dexamethasone (DEX) for 3 days to enhance epithelial barrier integrity. After measuring transepithelial electrical resistance (TER) and paracellular permeability, we extracted total RNA from 16HBE cells and performed microarray and pathway analysis. After we identified candidate genes and a canonical pathway, we measured TER and immunostained for tight junction (TJ) and adherent junction (AJ) proteins in cells that had been transfected with specific small interfering RNAs (siRNAs) for these genes. We identified a nuclear factor erythroid 2-related factor 2 (Nrf2)-mediated oxidative stress response pathway which was primarily involved in the steroid-induced enhancement of airway epithelial barrier integrity. Transfecting cells with Nrf2 specific siRNA reduced the steroid-induced enhancement of airway epithelial barrier integrity and the accumulation of TJ and AJ proteins at site...Continue Reading

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Citations

Feb 28, 2016·Archives of Toxicology·Mineko TeraoEnrico Garattini
Sep 1, 2016·The Journal of Allergy and Clinical Immunology·Nyall R LondonMurugappan Ramanathan
Sep 25, 2019·Drug Metabolism Reviews·Narges CheshmazarMaryam Hamzeh-Mivehroud
Jan 1, 2021·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·Elisabet JohanssonJocelyn M Biagini
Jun 18, 2017·Toxicology and Applied Pharmacology·Andres HenriquezUrmila P Kodavanti
May 8, 2021·Journal of Cellular and Molecular Medicine·Jiapeng HuYunxiao Shang
Jun 3, 2021·International Journal of Molecular Sciences·Renata Novak KujundžićKoraljka Gall Trošelj
Aug 7, 2019·Journal of Medicinal Chemistry·Nenad ManevskiFrancesca Toselli
Aug 28, 2021·Antioxidants·Munsoo HanTae Hoon Kim
Sep 10, 2021·Current Allergy and Asthma Reports·Evelyn M LelandMurugappan Ramanathan

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