Nuclear factor I (NFI) isoforms differentially activate simple versus complex NFI-responsive promoters.

The Journal of Biological Chemistry
A Z ChaudhryRichard M Gronostajski

Abstract

Promoter-specific differences in the function of transcription factors play a central role in the regulation of gene expression. We have measured the maximal transcriptional activation potentials of nuclear factor I (NFI) proteins encoded by each of the four identified NFI genes (NFI-A, -B, -C, and -X) by transient transfection in JEG-3 cells using two model NFI-dependent promoters: 1) a simple chimeric promoter containing a single NFI-binding site upstream of the adenovirus major late promoter (NFI-Ad), and 2) the more complex mouse mammary tumor virus long terminal repeat promoter. The relative activation potentials for the NFI isoforms differed between the two promoters, with NFI-X being the strongest activator of NFI-Ad and NFI-B being the strongest activator of the MMTV promoter. To determine if these promoter-specific differences in activation potential were due to the presence of glucocorticoid response elements (GREs), we added GREs upstream of the NFI-binding site in NFI-Ad. NFI-X remains the strongest activator of the GRE containing simple promoter, indicating that differences in relative activation potential are not due solely to the presence of GREs. Since NFI proteins bind to DNA as dimers, we assessed the activati...Continue Reading

References

Jun 1, 1991·Molecular and Cellular Biology·J J KnoxR M Gronostajski
Jan 1, 1990·Annual Review of Biochemistry·M Sawadogo, A Sentenac
Mar 1, 1990·Molecular and Cellular Biology·N GoyalR M Gronostajski
Mar 25, 1989·Nucleic Acids Research·G R MacGregor, C T Caskey
Jul 24, 1987·Nucleic Acids Research·R M Gronostajski
Oct 6, 1988·Nature·I SadowskiM Ptashne
Oct 20, 1988·Nature·M Ptashne
Aug 25, 1988·Nature·G Gill, M Ptashne
Nov 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·K NagataJ Hurwitz
Apr 1, 1995·Current Opinion in Genetics & Development·S J Triezenberg
Apr 26, 1994·Proceedings of the National Academy of Sciences of the United States of America·H AltmannE L Winnacker
May 10, 1994·Proceedings of the National Academy of Sciences of the United States of America·T K Kim, R G Roeder
Jul 1, 1993·Genes & Development·N Hernandez
Jun 15, 1996·Nucleic Acids Research·S WenzelidesE L Winnacker
Mar 1, 1997·Developmental Dynamics : an Official Publication of the American Association of Anatomists·A Z ChaudhryR M Gronostajski

❮ Previous
Next ❯

Citations

Feb 20, 2003·Biochimica Et Biophysica Acta·Li PanKenneth W Gross
Dec 17, 1998·The Biochemical Journal·K Yamada, T Noguchi
Dec 30, 2011·Journal of Virology·Bo LiangWilliam R Folk
Dec 4, 2003·Molecular and Cellular Biology·Cindy J BachurskiDihua Hong
Jan 6, 2005·Molecular and Cellular Biology·George Steele-PerkinsRichard M Gronostajski
Oct 26, 2005·BMC Developmental Biology·Elena LazakovitchRichard M Gronostajski
Dec 14, 2001·Biochemical and Biophysical Research Communications·K R SteffensenW Eskild
Oct 16, 1999·Proceedings of the National Academy of Sciences of the United States of America·L das NevesF Godinho
Nov 11, 2003·Proceedings of the National Academy of Sciences of the United States of America·Masahide ToneHerman Waldmann
Sep 24, 2008·Biochimica Et Biophysica Acta·Laura A Pérez-CasellasThomas A Linkhart
May 22, 2007·Biochemical and Biophysical Research Communications·Sudit S Mukhopadhyay, Jeffrey M Rosen
Jun 9, 2004·Biochemical and Biophysical Research Communications·Paul Delgado-OlguínRamón Coral-Vázquez
Dec 25, 2008·The Journal of Comparative Neurology·Asli KumbasarE David Litwack
Apr 17, 2015·BMC Cancer·Hye-Kyung LeeJoo-Cheol Park
Aug 24, 2017·Physiological Reports·Vasantha H S KumarRita M Ryan
May 6, 2003·Annals of Neurology·Conrad A MessamEugene O Major
Nov 16, 2019·American Journal of Medical Genetics. Part C, Seminars in Medical Genetics·Martin ZenkerRaoul C Hennekam
Mar 6, 2002·The Journal of Biological Chemistry·Marie Kannius-JansonJeanette Nilsson
Jun 7, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Rasoul Alikhani-KoupaeiFelix J Frey
Nov 18, 2000·Biochimica Et Biophysica Acta·M AndreaniG Béréziat

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.