Nuclear factor I represses the reverse-oriented transcription from the adenovirus type 5 DNA terminus.

Biochemical and Biophysical Research Communications
K MatsumotoM Ui

Abstract

The promoter activity of a cloned inverted terminal repeat (ITR) of human adenovirus (Ad) type 5 was found to be oriented in the opposite direction with respect to that of DNA replication in a cell-free transcription system using HeLa nuclear extracts. The major transcript was initiated outside the Ad sequence about 30 nucleotides downstream from the putative TATA-box located between nucleotide positions 9-18 of Ad 5 left terminus. Competitive transcription experiments using double-stranded oligonucleotides and isolated nuclear factor I revealed that nuclear factor I and its cognate binding site located upstream of the putative TATA-box are involved in negative regulation of the transcription from ITR promoter.

References

Dec 15, 1988·Biochemical and Biophysical Research Communications·T TamuraK Mikoshiba
Apr 8, 1988·Cell·L A ChodoshP A Sharp
May 25, 1988·Nucleic Acids Research·M MeisterernstE L Winnacker
May 1, 1985·Molecular and Cellular Biology·R M GronostajskiJ Hurwitz
Nov 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·K NagataJ Hurwitz
Oct 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·K NagataJ Hurwitz
May 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·R A GuggenheimerJ Hurwitz
Apr 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·F Tamanoi, B W Stillman
Aug 14, 2008·The Journal of Physical Chemistry. B·Loïc Leclercq, Andreea R Schmitzer

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Citations

Dec 14, 2001·Biochemical and Biophysical Research Communications·K R SteffensenW Eskild
Feb 11, 1992·Nucleic Acids Research·R J RoyS L Guérin
Jan 15, 1998·European Journal of Biochemistry·M J BergeronS L Guérin
Oct 29, 2010·International Journal of Hypertension·Sam L StephenSreenivasan Ponnambalam
Sep 4, 2008·Journal of Cellular Physiology·Cynthia St HilaireKatya Ravid
Jan 1, 1997·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·M A LanielS L Guérin

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