Nuclear factor-kappa B activation inhibits proliferation and promotes apoptosis of vascular smooth muscle cells.

Vascular
Lei JiaoMin Wu

Abstract

To investigate the role of nuclear factor-kappa B (NF-κB) performed in cell proliferation and apoptosis of vascular smooth muscle cells (VSMCs), and to assess the mechanisms. Human aorta VSMCs were divided into control, NF-κB inhibitor, NF-κB overexpression + NF-κB inhibitor, control vector + NF-κB inhibitor, NF-κB overexpression, and control vector groups. NF-κB overexpression vector was constructed and transfected into VSMCs. Proliferation of VSMCs in each group was detected by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide. Apoptosis of VSMCs was detected by flow cytometry. The expression of NF-κB, FasL, and hypertension-related gene (HRG-1) was measured by Western blotting. NF-κB overexpression vector was constructed correctly by restriction endonuclease, and the results showed that the activation of NF-κB could inhibit the proliferation of VSMCs. The results of flow cytometry also confirmed that NF-κB overexpression promoted apoptosis of VSMCs. Mechanically, NF-κB overexpression could up-regulate the expression of FasL and HRG-1. NF-κB overexpression promotes apoptosis and inhibits cell proliferation of VSMCs. The mechanisms might be regulated by promoting FasL and HRG-1 expression.

References

Apr 25, 2000·The Journal of Biological Chemistry·R W ClarksonC J Watson
Jul 17, 2001·Biochemical and Biophysical Research Communications·Z WangW H Newman
Dec 31, 2002·Journal of Molecular and Cellular Cardiology·Peter ZahradkaShawn Thomas
Apr 28, 2005·Virchows Archiv : an International Journal of Pathology·Xavier DolcetXavier Matias-Guiu
Dec 13, 2005·Journal of Pharmacological Sciences·Chieh-Hsi WuJui-Sung Hung
Oct 13, 2007·Experimental and Molecular Pathology·Caiying WangKaiyun Wu
Mar 23, 2012·Carcinogenesis·Malgorzata Magdalena SakInger Helene Madshus
Nov 13, 2012·The International Journal of Biochemistry & Cell Biology·Zhaoxia WangZhongdong Qiao
Jun 29, 2013·Chinese Medical Sciences Journal = Chung-kuo I Hsüeh K'o Hsüeh Tsa Chih·Li LiDe-pei Liu
Oct 9, 2015·Methods in Molecular Biology·Nichola L Figg, Martin R Bennett
Aug 5, 2016·Acta Neuropathologica Communications·Raman SagguGabor C Petzold
Aug 31, 2016·Journal of Endocrinological Investigation·M ErdoganA Berdeli
Nov 22, 2016·Insect Biochemistry and Molecular Biology·Anderson de Mendonça AmaranteMarcelo Rosado Fantappié
Dec 5, 2017·Critical Reviews in Oncology/hematology·Sam TilborghsPeter A van Dam
Apr 1, 2018·Biochemical and Biophysical Research Communications·Soyoung KwakYoon Seok Choi

❮ Previous
Next ❯

Methods Mentioned

BETA
transfection
electrophoresis
flow cytometry

Software Mentioned

SPSS
Quantity One
CellQuest Pro

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis