Nuclear factor-kappaB activation in human monocytes stimulated with lipopolysaccharide is inhibited by fibroblast conditioned medium and exogenous PGE2

FEBS Letters
Enrico ConteA Messina

Abstract

The nuclear factor kappaB (NF-kappaB) is thought to be crucially involved in the gene activation of several cytokines, including tumor necrosis factor alpha (TNF). Previously, we showed that fibroblast conditioned medium (FCM) is able to inhibit both TNF mRNA accumulation and protein release in peripheral blood-derived human monocytes (PBM) stimulated with lipopolysaccharide (LPS). In this study we have investigated the effect of FCM on the LPS-induced DNA-binding activity of NF-kappaB, by means of electrophoretic shift assay (EMSA). We provide evidence that FCM strongly inhibits the LPS-induced NF-kappaB activation in PBM. Furthermore, we show that exogenous PGE2 mimics the NF-kappaB inhibitory effect of FCM. On the other hand, FCM produced in the presence of indomethacin does not inhibit NF-kappaB activation by LPS. Our results lend further support to the hypothesis that inflammatory and immune responses of monocytes/macrophages may be modulated at the molecular level by signals originating from tissue structural cells such as fibroblasts.

References

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Citations

Dec 31, 2003·Trends in Immunology·Carlo VancheriNunzio Crimi
Dec 9, 2008·American Journal of Respiratory Cell and Molecular Biology·Marco FaillaCarlo Vancheri
Aug 23, 2011·American Journal of Physiology. Lung Cellular and Molecular Physiology·Ryan T DackorDarryl C Zeldin
Jan 5, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Weisong ZhouR Stokes Peebles
Jun 8, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·M SavardJ Gosselin
Dec 24, 2018·International Immunopharmacology·Xinwei HuangXiangyang Kong
Jul 24, 1998·Biochemical and Biophysical Research Communications·F SabouniS Semnanian
Feb 22, 2002·Experimental Cell Research·Takeshi ShimamuraBruce E Bejcek

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