Nuclear factor-kappaB is an important modulator of the altered gene expression profile and malignant phenotype in squamous cell carcinoma

Cancer Research
Amy LoercherCarter Van Waes

Abstract

We reported previously that transcription factor nuclear factor (NF)-kappaB is constitutively activated in human and murine squamous cell carcinomas (SCCs). The role of NF-kappaB in the cumulative changes in gene expression with transformation and progression of the murine SCC Pam 212 and after switching off NF-kappaB by a dominant negative inhibitor kappaB mutant (IkappaBalphaM) was explored by profiling with a 15,000-element cDNA micoarrray. Remarkably, NF-kappaB modulated the expression of >60% of the 308 genes differentially expressed between normal keratinocytes and metastatic SCCs. NF-kappaB directly or indirectly modulated expression of programs of genes functionally linked to proliferation, apoptosis, adhesion, and angiogenesis. Among these, changes in expression of cyclin D1, inhibitor of apoptosis-1, mutant Trp53, and beta-catenin detected with modulation of NF-kappaB by microarray were confirmed by Western and Northern blot. NF-kappaB DNA binding motifs were detected in the promoter of approximately 63% of genes showing increased expression and 33% of the genes showing decreased expression. The ACTACAG motif implicated in the NF-kappaB-dependent down-regulation of mRNA expression of MyoD and Sox9 was detected in the ...Continue Reading

References

Jan 31, 1998·The Journal of Clinical Investigation·M A SovakG E Sonenshein
May 9, 1998·Genes & Development·J Y ReutherA S Baldwin
Oct 22, 1998·Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery·T KatoC Van Waes
Jul 20, 1999·Molecular and Cellular Biology·D C GuttridgeA S Baldwin
Jan 27, 2000·Cell·D Hanahan, R A Weinberg
May 11, 2002·Nature Reviews. Cancer·Michael KarinZhi-Wei Li
Sep 5, 2002·Nature Reviews. Immunology·Ann Richmond

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Citations

May 18, 2005·The International Journal of Biochemistry & Cell Biology·An Van LaethemPatrizia Agostinis
May 11, 2013·Trends in Immunology·Rosalba SalcedoGiorgio Trinchieri
Nov 20, 2012·Nature Cell Biology·Arkasubhra GhoshVinay Tergaonkar
Aug 22, 2012·The Journal of Experimental Medicine·Christophe CataissonGiorgio Trinchieri
Oct 31, 2006·Omics : a Journal of Integrative Biology·Miroslav Blumenberg
Oct 24, 2009·Annals of Oncology : Official Journal of the European Society for Medical Oncology·C H ChungB A Murphy
Nov 7, 2009·Annals of Oncology : Official Journal of the European Society for Medical Oncology·B ZhouL Zhang
Jun 11, 2009·Japanese Journal of Clinical Oncology·Jong Gwang KimWansik Yu
May 3, 2005·Nucleic Acids Research·Lasse SinkkonenCarsten Carlberg
Oct 1, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Maie A St JohnSteven M Dubinett
Oct 1, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Pattatheyil ArunCarter Van Waes
Jun 3, 2005·Cancer Cell International·Mario BoccadoroJamie Cavenagh
Nov 15, 2007·PloS One·Savita AyyarPat Simpson
Oct 12, 2013·PloS One·Barbara HämmerleJaime Font de Mora
Nov 15, 2011·Critical Reviews in Eukaryotic Gene Expression·Sanjay L BhaveQuintin Pan
Aug 29, 2006·Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia·Alena SvobodovaJitka Vostalova
May 28, 2013·Journal of Skin Cancer·Kathryn E KingWendy C Weinberg
May 12, 2011·Expert Opinion on Therapeutic Targets·Chinnadurai Saravanan, Sushil Kumar Singh
Jun 19, 2012·Journal of Experimental & Clinical Cancer Research : CR·Vinita BatraHeather E Kleiner-Hancock
Jul 31, 2013·The Journal of Investigative Dermatology·Verena N LorenzCornelia S Seitz
Mar 5, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Sayantan BhattacharyyaNabendu Murmu
Aug 13, 2008·Expert Opinion on Therapeutic Targets·Matthew BrownCarter Van Waes

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