Nuclear factor kB activity in patients with acute severe cholangitis

World Journal of Gastroenterology : WJG
Jian-Ping GongXu-Hong Li

Abstract

To determine the NF-kB activity in peripheral blood mononuclear cells (PBMC) in patients with acute cholangitis of severe type (ACST) and correlate the degree of NF-kB activation with severity of biliary tract infection and clinical outcome. Twenty patients with ACST were divided into survivor group (13 cases) and nonsurvivor group (7 cases). Other ten patients undergoing elective gastrectomy or inguinal hernia repair were selected as control group. Peripheral blood samples were taken 24 hours postoperatively. PBMC were separated by density gradient centrifugation, then nuclear proteins were isolated from PBMC, and Electrophoretic Mobility Shift Assay (EMSA) used determined. The results were quantified by scanning densitometer of a Bio-Image Analysis System and expressed as relative optical density (ROD). The levels of TNF-alpha, IL-6, and IL-10 in the plasma of patients with ACST and healthy control subjects were determined by using an enzyme-linked immunoassay (ELISA). The NF-kB activity was 5.02 +/- 1.03 in nonsurvivor group, 2.98 +/- 0.51 in survivor group and 1.06 +/- 0.34 in control group. There were statistical differences in three groups (P<0.05). The levels of TNF-alpha and IL-6 in plasma were (498 +/- 53)ng.L(-1)and (...Continue Reading

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Citations

Jul 11, 2003·World Journal of Gastroenterology : WJG·Wei ZhouJie-Shou Li
Mar 31, 2004·World Journal of Gastroenterology : WJG·Jie SunJian-Guo Xu
Jan 1, 2003·World Journal of Gastroenterology : WJG·Bin TuSheng-Wei Li
Jan 27, 2006·World Journal of Gastroenterology : WJG·Ming-Qing XuLu-Nan Yan
Sep 3, 2013·Asian Pacific Journal of Tropical Biomedicine·Chuchard Punsawad
Mar 13, 2003·World Journal of Gastroenterology : WJG·Li-Li DaiChang-An Liu
Jan 13, 2004·World Journal of Gastroenterology : WJG·Wei WangBao-Ping Yu
Dec 26, 2008·World Journal of Gastroenterology : WJG·Tie-Cheng YangAi-Min Ren
Jan 9, 2010·American Journal of Physiology. Gastrointestinal and Liver Physiology·Katsutaka WatanabeMasato Nagino
Nov 1, 2006·World Journal of Gastroenterology : WJG·Xian LiAn Hong
Jun 5, 2002·World Journal of Gastroenterology : WJG·Jian-Ping GongXu-Hong Li
Oct 16, 2002·World Journal of Gastroenterology : WJG·Jian-Ping GongXu-Hong Li
Aug 30, 2014·Asian Pacific Journal of Cancer Prevention : APJCP·Hanaa Hibishy GaballahSaber Abdelrahman Ismail
Apr 2, 2002·World Journal of Gastroenterology : WJG·Jian-Ping GongYong Peng

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