PMID: 9648859Jul 2, 1998Paper

Nuclear factor of activated T cells (NFAT) as a new component of the signal transduction pathway in glioma cells

Journal of Neurochemistry
G MosieniakB Kaminska

Abstract

Cyclosporin A (CsA) exerts its immunosuppressive effect by inhibiting the activity of nuclear factor of activated T cells (NFAT), thus preventing transcriptional induction of several cytokine genes. This effect is mediated through inactivation of the phosphatase calcineurin, which inhibits translocation of an NFAT component to the nucleus. We have previously reported that CsA inhibits the growth of rat C6 glioma cells in a dose-dependent manner and induces apoptotic cell death. Here, we report that NFAT DNA-binding activity is present in the nuclear extracts from C6 glioma cells and that CsA treatment inhibits the formation of a functional NFAT complex. We provide evidence for the presence of a group of NFATc proteins in proliferating glioma cells. Immunoblot analyses show that stimulation of C6 glioma cells with a calcium-inducing agent, ionomycin, alters NFATc migration on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. This alteration is inhibited by simultaneous treatment with CsA, suggesting a calcineurin involvement in the regulation of glioma NFATc proteins. Direct immunofluorescence reveals the presence of NFATc proteins in nuclei of proliferating glioma cells and their disappearance in CsA-treated cells. The...Continue Reading

Citations

May 1, 2008·The European Journal of Neuroscience·José M Pérez-OrtizPedro Tranque
Sep 4, 2009·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Stephan BrechtWolfram F Neiss
Feb 6, 2002·The Journal of Biological Chemistry·Beata PyrzynskaBozena Kaminska
May 10, 2005·International Journal of Cancer. Journal International Du Cancer·Iwona CiechomskaBozena Kaminska
Apr 28, 2010·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Yan LiuHe-Long Zhang
Dec 24, 2009·Cancer Research·Srinivasulu ChigurupatiSic L Chan
Sep 29, 2011·Experimental and Molecular Pathology·Huiling LiuEddie Perkins
Apr 5, 2003·Molecular and Cellular Neurosciences·Malgorzata Zawadzka, Bozena Kaminska
Aug 28, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Aleksandra Ellert-MiklaszewskaBozena Kaminska

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