Nuclear GTP-binding proteins of Swiss 3T3 cells

FEBS Letters
S TakedaK Sekimizu

Abstract

The GTP-binding proteins of Swiss 3T3 cell nuclei were analyzed by filter binding assay and UV cross-linking analysis. The results showed the presence of multiple GTP-binding proteins in the nuclei. Scatchard analysis revealed that the Kd value for GTP binding to high-affinity components was 69 nM, that to low-affinity components being 2.7 microM. The GTP-binding activities of some nuclear proteins were found to change significantly in response to the growth conditions of the cells. During culture of cells in medium without serum, the GTP-binding activity of a 140 kDa protein clearly decreased, whereas that of a 40 kDa protein increased.

References

Jan 1, 1987·Annual Review of Biochemistry·A G Gilman
Dec 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·M T Franze-Fernández, A O Pogo

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Citations

Oct 1, 1992·Neurochemistry International·H OttoF Hucho
Jul 11, 2001·Prostaglandins & Other Lipid Mediators·B G MiguelA M Martínez
Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J B RubinsN Riedel
Aug 1, 1992·Seminars in Cell Biology·R F Irvine, N Divecha
Jun 26, 2020·Journal of Cellular Physiology·Chang GuoGuoyue Yuan

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