Nuclear heterogeneous nuclear ribonucleoprotein D is associated with poor prognosis and interactome analysis reveals its novel binding partners in oral cancer

Journal of Translational Medicine
Manish KumarR Ralhan

Abstract

Post-transcriptional regulation by heterogeneous ribonucleoproteins (hnRNPs) is an important regulatory paradigm in cancer development. Our proteomic analysis revealed hnRNPD overexpression in oral dysplasia as compared with normal mucosa; its role in oral carcinogenesis remains unknown. Here in we determined the hnRNPD associated protein networks and its clinical significance in oral squamous cell carcinoma (OSCC). Immunoprecipitation (IP) followed by tandem mass spectrometry was used to identify the binding partners of hnRNPD in oral cancer cell lines. Ingenuity pathway analysis (IPA) was carried out to unravel the protein interaction networks associated with hnRNPD and key interactions were confirmed by co-IP-western blotting. hnRNPD expression was analyzed in 183 OSCCs, 44 oral dysplasia and 106 normal tissues using immunohistochemistry (IHC) and correlated with clinico-pathological parameters and follow up data over a period of 91 months. Kaplan-Meier survival and Cox-multivariate-regression analyses were used to evaluate the prognostic significance of hnRNPD in OSCC. We identified 345 binding partners of hnRNPD in oral cancer cells. IPA unraveled novel protein-protein interaction networks associated with hnRNPD and sugges...Continue Reading

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Citations

Feb 10, 2016·Proteomics·Ajay MattaRanju Ralhan
May 22, 2016·Clinica Chimica Acta; International Journal of Clinical Chemistry·Manish KumarShyam Singh Chauhan
Nov 28, 2018·Wiley Interdisciplinary Reviews. RNA·Kiyoshi Masuda, Yuki Kuwano
Oct 22, 2021·Biochemical Pharmacology·Maria ReviejoJose J G Marin

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Datasets Mentioned

BETA
MDA1986

Methods Mentioned

BETA
immunoprecipitation
biopsies
PCR
electrophoresis
light microscopy
reverse-IP
co-IP

Software Mentioned

Proteinpilot
Ingenuity Pathway Analysis ( IPA )
Analyst QS
SPSS
IDA

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