Nuclear import of ho endonuclease utilizes two nuclear localization signals and four importins of the ribosomal import system

The Journal of Biological Chemistry
Anya BakhratDina Raveh

Abstract

Activity of Ho, the yeast mating switch endonuclease, is restricted to a narrow time window of the cell cycle. Ho is unstable and despite being a nuclear protein is exported to the cytoplasm for proteasomal degradation. We report here the molecular basis for the highly efficient nuclear import of Ho and the relation between its short half-life and passage through the nucleus. The Ho nuclear import machinery is functionally redundant, being based on two bipartite nuclear localization signals, recognized by four importins of the ribosomal import system. Ho degradation is regulated by the DNA damage response and Ho retained in the cytoplasm is stabilized, implying that Ho acquires its crucial degradation signals in the nucleus. Ho arose by domestication of a fungal VMA1 intein. A comparison of the primary sequences of Ho and fungal VMA1 inteins shows that the Ho nuclear localization signals are highly conserved in all Ho proteins, but are absent from VMA1 inteins. Thus adoption of a highly efficient import strategy occurred very early in the evolution of Ho. This may have been a crucial factor in establishment of homothallism in yeast, and a key event in the rise of the Saccharomyces sensu stricto.

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Citations

Sep 23, 2008·Current Genetics·Anya BakhratDina Raveh
Jul 1, 2010·BMC Cell Biology·Francisco José Taberner, Juan Carlos Igual
Sep 26, 2015·Biochimica Et Biophysica Acta·Leticia BaccariniPaula Portela
Oct 30, 2010·Biochimica Et Biophysica Acta·Yuh Min Chook, Katherine E Süel
Nov 26, 2009·FEMS Yeast Research·Bárbara Martínez-BonoJuan Carlos Igual
May 26, 2017·The Journal of Biological Chemistry·Fernando A Gonzales-ZubiateCarla Columbano Oliveira
Oct 25, 2008·Yeast·Keren Baranes-BacharDina Raveh
Jun 10, 2020·ELife·Laura N Rusche
Jun 20, 2020·The Journal of Biological Chemistry·Ellen K OkudaCarla C Oliveira
Jul 1, 2013·Comprehensive Reviews in Food Science and Food Safety·Indrani BhattacharyaR Y Surampalli

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