PMID: 8967456May 1, 1996Paper

Nuclear ion channel activity is regulated by actin filaments

The American Journal of Physiology
A G Prat, H F Cantiello

Abstract

Actin filaments are novel second messengers involved in ion channel regulation. Because cytoskeletal components interact with the nuclear envelope, the actin cytoskeleton may also control nuclear membrane function. In this report, the patch-clamp technique was applied to isolated nuclei from amphibian A6 epithelial cells to assess the role of actin filaments on nuclear ion channel activity under nucleus-attached or -excised conditions. The most prevalent spontaneous nuclear ion channel species, 76% (n = 46), was cation selective and had a maximal single-channel conductance of approximately 420 pS. Nuclear ion channels also displayed multiple subconductance states, including channel activity of 26 pS that was frequently observed. Nuclear ion channel activity on otherwise quiescent patches was induced by either addition of the actin cytoskeleton disrupter cytochalasin D (CD; 5 micrograms/ml, 60%, 3 of 5 patches) or actin (10-1,000 micrograms/ml) to the bathing solution of nucleus-attached patches (59%, 13 of 22 patches). Actin also induced ion channel activity in quiescent excised inside-out patches from the nuclear envelope (80%, 4 of 5 patches). In contrast, addition of bovine serum albumin (10-1,000 micrograms/ml) to the bathi...Continue Reading

References

Jan 1, 1992·Annual Review of Cell Biology·D J Forbes
Aug 1, 1992·Current Opinion in Cell Biology·L Gerace
Apr 1, 1991·The Journal of Membrane Biology·M MazzantiE F Smith
Jul 1, 1991·The Journal of Membrane Biology·L TabaresD E Clapham
Feb 22, 1990·Nature·M MazzantiH Malter
Mar 1, 1986·The Journal of Cell Biology·M Schindler, L W Jiang
Aug 1, 1981·Pflügers Archiv : European journal of physiology·O P HamillF J Sigworth
Feb 1, 1994·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M MazzantiM Rigatelli
Jan 1, 1993·The Journal of Membrane Biology·B Innocenti, M Mazzanti
Sep 1, 1993·The Journal of Cell Biology·N Panté, U Aebi
Jul 1, 1963·The Journal of General Physiology·W R LOEWENSTEIN, Y KANNO

❮ Previous
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Citations

Aug 12, 1998·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·J O Bustamante, W A Varanda
Aug 21, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A GuignandonL Vico
Feb 7, 2009·Nature Reviews. Molecular Cell Biology·Ning WangDonald E Ingber
Apr 20, 2012·PloS One·Yongyun Hwang, Abdul I Barakat
Jul 31, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·R ToniniM Mazzanti
Sep 5, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·V ShahinH Oberleithner
Nov 30, 2006·Annals of the New York Academy of Sciences·Max J Lab
Jul 28, 2016·Cellular and Molecular Bioengineering·Balázs Enyedi, Philipp Niethammer
Jan 12, 2001·Physiological Reviews·M MazzantiH Oberleithner
Mar 10, 1999·Progress in Biophysics and Molecular Biology·M J Lab
Jul 17, 2012·Journal of Cell Science·Akiko MammotoDonald E Ingber
May 28, 1998·Cell Calcium·M N BadmintonA K Campbell
Nov 11, 1999·Plant Physiology·A H van Der LuitA J Trewavas
Mar 21, 2007·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Kristopher M Garlick, R Meldrum Robertson
May 25, 2006·BMC Cell Biology·Sylvia MünterSpencer L Shorte
Aug 31, 2021·Current Opinion in Cell Biology·Guilherme Pedreira de Freitas NaderMatthieu Piel

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