PMID: 29755599May 15, 2018Paper

Nuclear Lamin Protein C Is Linked to Lineage-Specific, Whole-Cell Mechanical Properties

Cellular and Molecular Bioengineering
Rafael D González-CruzEric M Darling

Abstract

Lamin proteins confer nuclear integrity and relay external mechanical cues that drive changes in gene expression. However, the influence these lamins have on whole-cell mechanical properties is unknown. We hypothesized that protein expression of lamins A, B1, and C would depend on the integrity of the actin cytoskeleton and correlate with cellular elasticity and viscoelasticity. To test these hypotheses, we examined the protein expression of lamins A, B1, and C across five different cell lines with varied mechanical properties. Additionally, we treated representative "soft/stiff" cell types with cytochalasin D and LMNA siRNA to determine the effect of a more compliant whole-cell phenotype on lamin A, B1 and C protein expression. A positive, linear correlation existed between lamin C protein expression and average cell moduli/apparent viscosity. Though moderate correlations existed between lamin A/B1 protein expression and whole-cell mechanical properties, they were statistically insignificant. Inhibition of actin polymerization, via cytochalasin D treatment, resulted in reduced cell elasticity, viscoelasticity, and lamin A and C protein expression in "stiff" MG-63 cells. In "soft" HEK-293T cells, this treatment reduced cell ela...Continue Reading

References

Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·D Z FisherG Blobel
Apr 20, 2002·Biophysical Journal·Emilios K DimitriadisRichard S Chadwick
Jul 3, 2004·Proceedings of the National Academy of Sciences of the United States of America·Laurent VergnesKaren Reue
Sep 24, 2005·Stem Cells·Dan ConstantinescuAntonei B Csoka
Feb 16, 2006·Osteoarthritis and Cartilage·E M DarlingF Guilak
Mar 3, 2006·The Journal of Clinical Investigation·Loren G FongStephen G Young
Jul 11, 2006·The Journal of Biological Chemistry·Jan LammerdingRichard T Lee
May 10, 2007·The Journal of Biological Chemistry·Julie Y JiJan Lammerding
Jun 2, 2007·Acta Biomaterialia·Subra Suresh
Mar 4, 2008·Nature Cell Biology·Paola Scaffidi, Tom Misteli
Apr 9, 2008·Experimental Cell Research·P J Stewart-HutchinsonDidier Hodzic
Jun 7, 2008·Circulation Research·Kris Noel DahlJan Lammerding
Jul 26, 2008·Nature Nanotechnology·Sarah E CrossJames K Gimzewski
Oct 14, 2008·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Rahima AkterGustavo Duque
Dec 24, 2010·Current Opinion in Cell Biology·Jason A MelladCatherine M Shanahan
Feb 7, 2012·Proceedings of the National Academy of Sciences of the United States of America·Hea-Jin JungLoren G Fong
May 23, 2012·Proceedings of the National Academy of Sciences of the United States of America·Rafael D González-CruzEric M Darling
Jun 7, 2012·Journal of Cell Science·Chin Yee Ho, Jan Lammerding
Nov 6, 2013·Proceedings of the National Academy of Sciences of the United States of America·Jae-Won ShinDennis E Discher
Feb 26, 2014·The Journal of Cell Biology·Takamasa HaradaDennis E Discher
Jun 26, 2014·Journal of Cell Science·Joe Swift, Dennis E Discher
Aug 16, 2014·Current Biology : CB·Amnon BuxboimDennis E Discher
Apr 9, 2015·Science Signaling·Kevin A Janes
Apr 23, 2015·Proceedings of the National Academy of Sciences of the United States of America·Srujana NeelamTanmay P Lele
Jun 25, 2015·Proceedings of the National Academy of Sciences of the United States of America·Francois BordeleauCynthia A Reinhart-King
Dec 25, 2015·Proceedings of the National Academy of Sciences of the United States of America·Ekta MakhijaG V Shivashankar
Mar 22, 2016·The Journal of Clinical Investigation·John M LeeLoren G Fong
Jun 16, 2016·Scientific Reports·Manindra BeraKaushik Sengupta
Aug 30, 2016·Cellular and Molecular Bioengineering·Jerome IriantoDennis E Discher
May 10, 2017·Journal of Biophotonics·Thorsten KolbGraeme Whyte

❮ Previous
Next ❯

Citations

Nov 7, 2019·Emerging Topics in Life Sciences·Yuntao XiaJerome Irianto
Nov 20, 2018·Frontiers in Cell and Developmental Biology·Rafael D González-CruzEric M Darling

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.