Mar 19, 2015

Nuclear lamins are not required for lamina-associated domain organization in mouse embryonic stem cells

EMBO Reports
Mario Amendola, Bas van Steensel

Abstract

In mammals, the nuclear lamina interacts with hundreds of large genomic regions, termed lamina-associated domains (LADs) that are generally in a transcriptionally repressed state. Lamins form the major structural component of the lamina and have been reported to bind DNA and chromatin. Here, we systematically evaluate whether lamins are necessary for the LAD organization in murine embryonic stem cells. Surprisingly, removal of essentially all lamins does not have any detectable effect on the genome-wide interaction pattern of chromatin with emerin, a marker of the inner nuclear membrane. This suggests that other components of the lamina mediate these interactions.

  • References36
  • Citations39

Citations

Mentioned in this Paper

Lamins
Biological Markers
Inner Nuclear Membrane
Leukocyte Adhesion Deficiency Type 1
Genome
Nuclear Lamina
Blade - plant part
Murine
Lamiinae (invertebrate)
Cell Nucleus

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