PMID: 8953247Nov 1, 1996Paper

Nuclear localization activity of phytochrome B

The Plant Journal : for Cell and Molecular Biology
K Sakamoto, A Nagatani

Abstract

Phytochromes are soluble red/far-red-light photoreceptor proteins which mediate various photomorphogenic responses of plants. Despite much effort, the signal transduction mechanism of phytochrome has remained obscure. Phytochromes are encoded by a small multigene family in Arabidopsis. Among the members of the family, phytochrome A (phyA) and B (phyB) are the best characterized. PhyB contains putative nuclear localization signals within its C-terminal region. Transgenic Arabidopsis plants were produced with expressed a fusion protein consisting of GUS and C-terminal fragments of phyB. GUS staining from the fusion protein in these transgenic plants was observed in the nucleus, which suggests that the nuclear localization signal of the fragment is functional. Next, it was examined whether the endogenous phyB was detected in the nucleus. Nuclei were isolated from the light-grown wild-type Arabidopsis leaves and subjected to the immunoblot analysis. The result indicated that a substantial fraction of total phyB was recovered in the isolated nuclei. This result was further confirmed by the immunocytochemical analysis of the protoplasts. Finally, the effects of light treatments on the levels of phyB in the isolated nuclei were examin...Continue Reading

Citations

Mar 1, 1997·Journal of Plant Research·A Nagatani
Apr 1, 2008·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Laju K Paul, Jitendra P Khurana
Aug 4, 1999·Trends in Plant Science·H Frohnmeyer
Nov 14, 2000·Current Opinion in Plant Biology·F Nagy, E Schäfer
Mar 6, 1999·Current Opinion in Plant Biology·A Nagatani
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Oct 6, 1997·The EMBO Journal·A C Mustilli, C Bowler
Jul 29, 1998·The Plant Journal : for Cell and Molecular Biology·E GendreauJ Traas
Sep 4, 1999·The Plant Journal : for Cell and Molecular Biology·O KleinerA Batschauer
Jun 14, 2002·The Plant Journal : for Cell and Molecular Biology·Young-Mi KimMoon-Soo Soh
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Apr 22, 2009·Proceedings of the National Academy of Sciences of the United States of America·Jieun ShinGiltsu Choi
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