Nuclear Localization of Robo is Associated with Better Survival in Bladder Cancer

Pathology Oncology Research : POR
Ulrich KrafftTibor Szarvas

Abstract

The Slit-Robo pathway has shown to be altered in several malignant diseases. However, its role in bladder cancer is poorly understood. Therefore, we aimed to assess the tissue expression of Robo1 and Robo4 as well as their ligand Slit2 in different stages of bladder cancer to explore possible changes of Slit-Robo signalling during the progression of bladder cancer. Robo1, Robo4 and Slit2 gene expressions were analyzed in 92 frozen bladder cancer tissue samples by using reverse transcription quantitative real-time PCR. Immunohistochemical analyses were performed on 149 formalin-fixed and paraffin-embedded bladder cancer tissue samples. Results were correlated with the clinical and follow-up data by performing both univariable and multivariable analyses. Robo1 and Robo4 nuclear staining intensitiy was significantly higher in low stage and low grade bladder cancer. Elevated Robo1 nuclear staining was associated with better disease-specific survival (DSS) (p = 0.045). Similarly, stronger Robo4 nuclear staining tended to be associated with longer DSS (p = 0.061). We found higher Robo1 and Slit2 gene expression levels in advanced stages of bladder cancer (p = 0.007 and p < 0.001). High Slit2 gene expression was correlated with signif...Continue Reading

References

Jan 1, 1995·Annals of Oncology : Official Journal of the European Society for Medical Oncology·V SundaresanP Rabbitts
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Nov 10, 2004·British Journal of Cancer·R E DickinsonF Latif
Feb 17, 2006·The Journal of Biological Chemistry·Sukhbir KaurRamani Ramchandran
Mar 7, 2006·World Journal of Urology·John P Stein, Donald G Skinner
May 27, 2006·Advances in Health Sciences Education : Theory and Practice·Timothy J WoodGeoffrey R Norman
Feb 3, 2007·Breast Cancer Research and Treatment·Bernd C SchmidRobert Zeillinger
Jul 10, 2008·The Journal of Biological Chemistry·Anil PrasadRamesh K Ganju
Oct 3, 2008·Cancer Research·Rebecca MarlowLindsay Hinck
Nov 14, 2008·Molecular Biology of the Cell·Maria Cristina StellaPaolo M Comoglio
Jan 27, 2010·Reproduction : the Official Journal of the Society for the Study of Fertility·Rachel E Dickinson, W Colin Duncan
Oct 30, 2012·Nature·Andrew V BiankinSean M Grimmond
Sep 24, 2014·Drug Discovery Today·Rishi K GaraSubhash C Chauhan
Oct 11, 2017·Cell·A Gordon RobertsonSeth P Lerner

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Methods Mentioned

BETA
PCR
electrophoresis
nuclear translocation

Software Mentioned

Slit
OncoLnc
Robo

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