PMID: 9188558Jul 1, 1997Paper

Nuclear localization of the NS3 protein of hepatitis C virus and factors affecting the localization

Journal of Virology
S MuramatsuHak Hotta

Abstract

Subcellular localization of the NS2 and NS3 proteins of hepatitis C virus was analyzed. In stable Ltk transfectants inducibly expressing an NS2-NS3 polyprotein (amino acids [aa] 810 to 1463), processed full-size NS2 (aa 810 to 1026) was detected exclusively in a cytoplasmic membrane fraction. On the other hand, the other processed product, carboxy-truncated NS3 (NS3 deltaC1463; aa 1027 to 1463), was present in both cytoplasmic and nuclear fractions. To further analyze subcellular localization of NS3, NS3 deltaC1459 (aa 1027 to 1459), full-size NS3 (NS3F; aa 1027 to 1657), and both amino- and carboxy-truncated NS3 (NS3 deltaNdeltaC; aa 1201 to 1459) were expressed in HeLa cells by using a vaccinia virus-T7 hybrid expression system. NS3 deltaC1459 and NS3F accumulated in the nucleus as well as in the cytoplasm, exhibiting a dot-like staining pattern. On the other hand, NS3 deltaNdeltaC was localized predominantly in the cytoplasm, suggesting the presence of a nuclear localization signal(s) in the amino-terminal sequence of NS3. NS4A, a viral cofactor for the NS3 protease, inhibited nuclear transport of NS3 deltaC1459 and NS3F, with the latter inhibited to a lesser extent than was the former. Interestingly, wild-type p53 tumor sup...Continue Reading

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Citations

Jul 1, 1999·Protein Science : a Publication of the Protein Society·A Y HoweJ Y Lau
Jan 5, 2002·Microbiology and Immunology·I YoshidaH Hotta
Mar 14, 2009·The Journal of General Virology·Yasuaki BungyokuHak Hotta
Jun 29, 2004·Journal of Medical Virology·Krister MelénIlkka Julkunen
Apr 26, 2007·Folia Microbiologica·L KrekulováL W Riley
Jul 10, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·D MoradpourH E Blum
Oct 17, 2001·The Journal of General Virology·B GrummerI Greiser-Wilke
Jul 7, 2005·Liver International : Official Journal of the International Association for the Study of the Liver·Aldona KasprzakMaciej Zabel
Sep 27, 2007·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Aldona Kasprzak, Agnieszka Adamek
Feb 7, 1998·European Journal of Biochemistry·Y GwackJ Choe
Mar 9, 2021·Archives of Virology·Selvin Noé Palacios-RápaloRosa María Del Ángel
Jan 31, 2019·Nucleic Acids Research·Shira PerezMeital Gal-Tanamy
Jun 30, 2000·Biochemical and Biophysical Research Communications·F WangH Hotta
Mar 26, 1998·Biochemical and Biophysical Research Communications·S IshidoH Hotta
Jul 16, 1999·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·P BorowskiM Heiland
Aug 25, 2012·Biochemistry·Veronica M RaneyKevin D Raney

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