PMID: 9551185Jan 1, 1997Paper

Nuclear localization signals: a driving force for nuclear transport of plasmid DNA in zebrafish

Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire
P Collas, P Aleström

Abstract

Nuclear localization signals (NLSs) are short peptides required for nuclear transport of karyophilic proteins. We review in this paper how the nuclear targeting property of NLS peptides has been taken advantage of to enhance the efficiency of nuclear uptake of transgene DNA in zebrafish and how it may improve the efficiency of transgenesis in this species. Synthetic NLS peptides can bind to plasmid DNA by ionic interactions. Cytoplasmic injection of DNA-NLS complexes in zebrafish eggs enhances the rate and the amount of plasmid DNA taken up by embryonic nuclei. Nuclear import of DNA-NLS complexes has been duplicated in vitro and exhibits energetic and cytosolic requirements similar to those for nuclear protein import. Furthermore, binding NLSs to DNA increases expression frequency of the transgene. We suggest that NLS peptides may constitute a valuable tool to improve the efficiency of transgenesis in zebrafish and other species.

Citations

Dec 1, 2013·Drug Delivery and Translational Research·Rajan SwamiSistla Ramakrishna
Dec 31, 2008·Chembiochem : a European Journal of Chemical Biology·Fozia NoorNils Metzler-Nolte
Oct 7, 2015·Journal of Controlled Release : Official Journal of the Controlled Release Society·Samuel T Crowley, Kevin G Rice
Dec 10, 1999·Experimental Cell Research·D A DeanL C Smith
Nov 22, 2005·Expert Opinion on Drug Delivery·Rui ZhouDavid A Dean
Jun 26, 2001·Molecular Reproduction and Development·S MangosG M Kelly
Mar 10, 2006·Pharmaceutical Research·Marieke A E M van der AaDaan J A Crommelin

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