PMID: 12775045May 31, 2003Paper

Nuclear magnetic resonance and diffuse-reflectance infrared Fourier transform spectroscopy of biosolids-derived biocolloidal organic matter

Environmental Science & Technology
J D MaoM L Thompson

Abstract

We extracted the acid-soluble portion of municipal biosolids, fractionated it by both molecular weight (MW) and hydrophobicity, and used various solid-state nuclear magnetic resonance (NMR) methods and diffuse-reflectance infrared Fourier transform (DRIFT) spectroscopy to characterize the fractions. Spectroscopic characterization of the MW components of the biosolids-derived organic matter fractions revealed the presence of functionally distinct groups of compounds. Quantitative 13C NMR, CH spectral editing, and several two-dimensional NMR experiments show that the high-MW hydrophilic fraction in particular is structurally simple, consisting predominantly of N-acetylated polysaccharides, perhaps derived from bacterial peptidoglycans. In the high-MW hydrophobic fraction, aromatic compounds were present in addition to the N-acetylated polysaccharides. Infrared spectroscopy confirmed that hydrophilic fractions were dominated by carbohydrates and indicated that the lower-MW fractions lacked amide moieties. Complementary interpretations of the DRIFT and NMR spectra improved our knowledge of the components separated by this fractionation scheme, allowing better characterization of biosolids organic matter. Moreover, fractionation bas...Continue Reading

Citations

Jun 25, 2008·Journal of Environmental Quality·Charisma LattaoRobert L Cook
Jun 23, 2018·The Science of the Total Environment·Kun WangYantao Song
Feb 7, 2018·Progress in Nuclear Magnetic Resonance Spectroscopy·Jun KikuchiYasuhiro Date
May 30, 2017·Progress in Nuclear Magnetic Resonance Spectroscopy·Jingdong MaoKlaus Schmidt-Rohr

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