Nuclear magnetic resonance characterization of the N-terminal thioredoxin-like domain of protein disulfide isomerase

Protein Science : a Publication of the Protein Society
J KemminkT E Creighton

Abstract

A genetically engineered protein consisting of the 120 residues at the N-terminus of human protein disulfide isomerase (PDI) has been characterized by 1H, 13C, and 15N NMR methods. The sequence of this protein is 35% identical to Escherichia coli thioredoxin, and it has been found also to have similar patterns of secondary structure and beta-sheet topology. The results confirm that PDI is a modular, multidomain protein. The last 20 residues of the N-terminal domain of PDI are some of those that are similar to part of the estrogen receptor, yet they appear to be an intrinsic part of the thioredoxin fold. This observation makes it unlikely that any of the segments of PDI with similarities to the estrogen receptor comprise individual domains.

References

Nov 1, 1992·Journal of Biomolecular NMR·M PiottoV Sklenár
Nov 20, 1991·Journal of Molecular Biology·D S WishartF M Richards
Mar 5, 1990·Journal of Molecular Biology·S K KattiH Eklund
May 1, 1990·Quarterly Reviews of Biophysics·S W Fesik, E R Zuiderweg
Jan 1, 1985·Progress in Biophysics and Molecular Biology·W G Hol
Jan 1, 1985·Annual Review of Biochemistry·A Holmgren
Aug 1, 1994·Trends in Biochemical Sciences·R B FreedmanM F Tuite
May 1, 1993·Protein Science : a Publication of the Protein Society·M Wunderlich, R Glockshuber

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Citations

Apr 3, 1998·Matrix Biology : Journal of the International Society for Matrix Biology·K I Kivirikko, J Myllyharju
May 30, 2009·Antioxidants & Redox Signaling·Feras Hatahet, Lloyd W Ruddock
Sep 21, 2010·Antioxidants & Redox Signaling·Matthieu DepuydtJean-Francois Collet
Feb 3, 2005·The Journal of Peptide Research : Official Journal of the American Peptide Society·C RennerL Moroder
Apr 17, 2012·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Shoko HashimotoSusumu Imaoka
Feb 10, 2009·Biophysical Chemistry·Janet S AndersonDavid M LeMaster
Jan 20, 2009·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Francisco Vargas-AlboresFlorinda Jiménez-Vega
Aug 16, 2005·Progress in Biophysics and Molecular Biology·Alexandra P CarvalhoMaria J Ramos
Apr 8, 2015·Proceedings of the National Academy of Sciences of the United States of America·Anna KaplanBrent R Stockwell
Jun 18, 1998·Folding & Design·M Huber-Wunderlich, R Glockshuber
Feb 28, 2008·Chembiochem : a European Journal of Chemical Biology·Griselda HernándezDavid M LeMaster
Apr 7, 2000·Protein Science : a Publication of the Protein Society·F VinciG Marino
Oct 13, 2006·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Rehab F AbdelhamidHiroshi Hori
Nov 5, 1997·The Journal of Biological Chemistry·Y Dai, C Wang
Jan 31, 2013·Chemical Communications : Chem Comm·Samantha L TaylorMark J Howard
Apr 1, 1997·Journal of Bacteriology·D Missiakas, S Raina
Aug 28, 2021·Nanomaterials·Xiaoqing ZengDeqiang Wang
Jul 21, 2009·International Journal of Biological Macromolecules·Carlos NovoAna Armada

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