Nuclear magnetic resonance characterization of the myristoylated, N-terminal fragment of ADP-ribosylation factor 1 in a magnetically oriented membrane array

Biochemistry
J A Losonczi, J H Prestegard

Abstract

The behavior of the N-terminal fragment of human ADP-ribosylation factor 1 (ARF1) in a membranelike environment is described. This is accomplished using heteronuclear liquid crystal NMR techniques in a magnetically oriented membrane array on a selectively 13C- and 15N-labeled peptide. After full assignment of the labeled sites, residual dipolar couplings (13C-13C, 15N-1H and, 13C-15N) and chemical shift anisotropy effects (amide 13C and 15N) were measured. The experimental data were interpreted using order matrix calculations to yield orientational and dynamic information for four separate, rigid amide planes. The experimental data obtained proves that the amphipathic peptide interacts with the bilayer in a mode that is consistent with an alpha-helix having its axis parallel to the membrane surface. Possibilities of extending the employed techniques to larger and uniformly labeled systems are discussed.

Citations

Oct 28, 1998·Biophysical Journal·P O Quist
Feb 13, 2001·Chemistry and Physics of Lipids·V ChupinB de Kruijff
Feb 19, 2003·Journal of Molecular Biology·Scott A McCallum, Arthur Pardi
Mar 19, 2003·Bioorganic Chemistry·Jennifer A WhilesEdward A Dennis
Jan 12, 2002·Current Opinion in Structural Biology·J R Tolman
Jan 12, 2002·Current Opinion in Structural Biology·A Arora, L K Tamm
Nov 18, 1998·Current Opinion in Structural Biology·F M Marassi, S J Opella
Jul 17, 1998·Nature Structural Biology·J H Prestegard
Jul 6, 2010·Nature Structural & Molecular Biology·Yizhou LiuJames H Prestegard
Mar 4, 2000·Proceedings of the National Academy of Sciences of the United States of America·O VinogradovaJ Qin
Nov 5, 2011·The Journal of Biological Chemistry·Geok-Lin ChuaSurajit Bhattacharjya
Jun 5, 2012·The Journal of Biological Chemistry·Jung Chae LimRodney L Levine
Oct 29, 2005·Nucleic Acids Research·Anita I KishoreJames H Prestegard
Dec 2, 2004·Molecular and Cellular Biology·Séverine Celton-MorizurAnne Paoletti
Aug 31, 2000·Analytical Biochemistry·L Czerski, C R Sanders
Dec 4, 2004·Magnetic Resonance in Chemistry : MRC·Jiangli Yan, Edward R Zartler
Sep 8, 2010·Natural Product Reports·Nobuaki Matsumori, Michio Murata
Aug 28, 2012·Chemical Reviews·Ulrich H N DürrAyyalusamy Ramamoorthy
Jan 5, 2000·Biophysical Journal·J StruppeR R Vold
Jan 27, 2005·Biochimica Et Biophysica Acta·Thad A HarrounJohn Katsaras
Apr 12, 2011·Biochimica Et Biophysica Acta·Dror E WarschawskiIsabelle Marcotte
Sep 5, 2001·Journal of Magnetic Resonance·M L MangelsG A Lorigan
Jan 5, 2000·Journal of Magnetic Resonance·H M Al-HashimiJ H Prestegard
Oct 22, 1998·Structure·C R Sanders, R S Prosser
Jan 15, 2009·Structure·Yizhou LiuJames H Prestegard
Oct 28, 1998·Biophysical Journal·R S ProsserI V Shiyanovskaya
Feb 13, 2001·Biophysical Journal·J A WhilesR R Vold
May 26, 1999·Journal of Magnetic Resonance·J A LosoncziJ H Prestegard
Apr 6, 2005·Methods in Enzymology·Peter J Lukavsky, Joseph D Puglisi
Jul 28, 2016·Small GTPases·Agata NawrotekJacqueline Cherfils
May 26, 1999·Journal of Molecular Biology·M GeyerH R Kalbitzer
May 2, 2000·The Journal of Biological Chemistry·X ZhuR A Kahn
Dec 19, 2018·Biochemical Society Transactions·François PeuroisJacqueline Cherfils
Sep 30, 1999·Molecular Membrane Biology·J E Johnson, R B Cornell
Apr 23, 1999·The Journal of Biological Chemistry·G Pacheco-RodriguezM Vaughan

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