Nuclear magnetic resonance evidence for the dimer formation of beta amyloid peptide 1-42 in 1,1,1,3,3,3-hexafluoro-2-propanol

Analytical Biochemistry
Yoshiki ShigemitsuHidekazu Hiroaki

Abstract

Alzheimer's disease involves accumulation of senile plaques in which filamentous aggregates of amyloid beta (Aβ) peptides are deposited. Recent studies demonstrate that oligomerization pathways of Aβ peptides may be complicated. To understand the mechanisms of Aβ(1-42) oligomer formation in more detail, we have established a method to produce (15)N-labeled Aβ(1-42) suited for nuclear magnetic resonance (NMR) studies. For physicochemical studies, the starting protein material should be solely monomeric and all Aβ aggregates must be removed. Here, we succeeded in fractionating a "precipitation-resistant" fraction of Aβ(1-42) from an "aggregation-prone" fraction by high-performance liquid chromatography (HPLC), even from bacterially overexpressed Aβ(1-42). However, both Aβ(1-42) fractions after 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) treatment formed amyloid fibrils. This indicates that the "aggregation seed" was not completely monomerized during HFIP treatment. In addition, Aβ(1-42) dissolved in HFIP was found to display a monomer-dimer equilibrium, as shown by two-dimensional (1)H-(15)N NMR. We demonstrated that the initial concentration of Aβ during the HFIP pretreatment altered the kinetic profiles of Aβ fibril formation in a...Continue Reading

References

Jan 22, 2003·Biochimica Et Biophysica Acta·Euridice P VieiraHelmuth Möhwald
Aug 29, 2003·Journal of Biochemistry·Nami Hirota-NakaokaYuji Goto
Mar 12, 2009·Journal of the American Chemical Society·Naoyuki MiyashitaYuji Sugita
Feb 4, 2011·Chembiochem : a European Journal of Chemical Biology·Muralidhar DasariBernd Reif
Aug 20, 2011·Nature Reviews. Drug Discovery·Eric KarranBart De Strooper
Jan 19, 2012·Journal of Peptide Science : an Official Publication of the European Peptide Society·Sanjai Kumar PachaharaRamakrishnan Nagaraj
May 16, 2012·FEBS Letters·Kirill D NadezhdinAlexander S Arseniev
Aug 1, 2015·Proceedings of the National Academy of Sciences of the United States of America·Takayuki OhnishiMinako Hoshi

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Citations

Aug 18, 2018·International Journal of Molecular Sciences·Daniel Miguel Ángel Villalobos AcostaMartha Cecilia Rosales Hernández
Jun 15, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Kazuma Murakami, Kazuhiro Irie
Jan 4, 2019·Current Protein & Peptide Science·Botond PenkeLívia Fülöp
Oct 11, 2017·Journal of Biochemistry·Yoshiki Shigemitsu, Hidekazu Hiroaki
Mar 5, 2021·PloS One·Maruf Mohammad AkborMasaharu Isobe
Jun 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Anatoly S UrbanEduard V Bocharov

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