Nuclear Magnetic Resonance Solution Structures of Lacticin Q and Aureocin A53 Reveal a Structural Motif Conserved among Leaderless Bacteriocins with Broad-Spectrum Activity

Biochemistry
Jeella Z AcedoJohn C Vederas

Abstract

Lacticin Q (LnqQ) and aureocin A53 (AucA) are leaderless bacteriocins from Lactococcus lactis QU5 and Staphylococcus aureus A53, respectively. These bacteriocins are characterized by the absence of an N-terminal leader sequence and are active against a broad range of Gram-positive bacteria. LnqQ and AucA consist of 53 and 51 amino acids, respectively, and have 47% identical sequences. In this study, their three-dimensional structures were elucidated using solution nuclear magnetic resonance and were shown to consist of four α-helices that assume a very similar compact, globular overall fold (root-mean-square deviation of 1.7 Å) with a highly cationic surface and a hydrophobic core. The structures of LnqQ and AucA resemble the shorter two-component leaderless bacteriocins, enterocins 7A and 7B, despite having low levels of sequence identity. Homology modeling revealed that the observed structural motif may be shared among leaderless bacteriocins with broad-spectrum activity against Gram-positive organisms. The elucidated structures of LnqQ and AucA also exhibit some resemblance to circular bacteriocins. Despite their similar overall fold, inhibition studies showed that LnqQ and AucA have different antimicrobial potency against t...Continue Reading

References

Jun 1, 1995·Journal of Bacteriology·R W WoroboM E Stiles
Sep 19, 1994·FEBS Letters·B SamynJ Van Beeumen
Nov 1, 1995·Journal of Biomolecular NMR·F DelaglioA Bax
Nov 21, 1997·Journal of Molecular Biology·P GüntertK Wüthrich
Jun 6, 2000·American Journal of Physiology. Cell Physiology·J I Kourie, A A Shorthouse
Sep 27, 2000·Proceedings of the National Academy of Sciences of the United States of America·C GonzálezM Rico
Jun 11, 2002·Journal of Molecular Biology·Daili Jacqueline Aguilar NetzHans-Georg Sahl
Oct 31, 2002·Applied and Environmental Microbiology·Daili Jacqueline Aguilar NetzHans-Georg Sahl
Jun 25, 2004·Nucleic Acids Research·Todd J DolinskyNathan A Baker
Aug 20, 2004·Methods in Molecular Biology·Bruce A Johnson
Nov 23, 2005·Bioinformatics·Konstantin ArnoldTorsten Schwede
Feb 8, 2007·Proceedings of the National Academy of Sciences of the United States of America·Dzung B DiepIngolf F Nes
Aug 11, 2007·Bioscience, Biotechnology, and Biochemistry·Shun IwataniKenji Sonomoto
Nov 18, 2008·Applied and Environmental Microbiology·Fuminori YoneyamaKenji Sonomoto
May 28, 2009·Antimicrobial Agents and Chemotherapy·Fuminori YoneyamaKenji Sonomoto
Aug 21, 2009·The Journal of Biological Chemistry·Leah A Martin-VisscherJohn C Vederas
Jan 9, 2010·Bioscience, Biotechnology, and Biochemistry·Fuminori YoneyamaKenji Sonomoto
Apr 8, 2011·Journal of Agricultural and Food Chemistry·Xiaoji LiuMarco J van Belkum
Dec 14, 2011·Journal of Bacteriology·Janaína Dos Santos NascimentoMaria do Carmo de Freire Bastos
Dec 22, 2011·International Journal of Microbiology·Christopher T Lohans, John C Vederas
Feb 22, 2012·Beneficial Microbes·Y MasudaK Sonomoto
Jan 22, 2013·Applied and Environmental Microbiology·Shun IwataniKenji Sonomoto
Jun 5, 2013·Antimicrobial Agents and Chemotherapy·Mengqi LiKenji Sonomoto
Feb 28, 2014·Future Microbiology·Timothy D ArthurMichael L Chikindas
Jul 6, 2014·The Journal of Biological Chemistry·Kirill V OvchinnikovDzung B Diep
Jul 22, 2014·Journal of Peptide Science : an Official Publication of the European Peptide Society·Susmita BandyopadhyayChiradip Chatterjee
Dec 17, 2014·Current Protocols in Bioinformatics·Fabian Sievers, Desmond G Higgins
Feb 15, 2015·Applied and Environmental Microbiology·Jeella Z AcedoJohn C Vederas
Apr 18, 2015·Nucleic Acids Research·Alexey DrozdetskiyGeoffrey J Barton
Aug 13, 2015·Current Topics in Medicinal Chemistry·Hashem EtayashKamaljit Kaur
Sep 6, 2015·International Journal of Antimicrobial Agents·Veronica L CaveraMichael L Chikindas
Dec 1, 2010·Probiotics and Antimicrobial Proteins·Marco J van BelkumJohn C Vederas

❮ Previous
Next ❯

Citations

Aug 8, 2018·FEMS Microbiology Reviews·Jeella Z AcedoMarco J van Belkum
Feb 16, 2019·Current Protein & Peptide Science·Marian VincenziMarilisa Leone
Sep 21, 2018·Frontiers in Microbiology·Rodney H PerezKenji Sonomoto
Nov 1, 2020·Applied Microbiology and Biotechnology·Maria do Carmo de Freire BastosMarcus Lívio Varella Coelho
May 18, 2021·Frontiers in Molecular Biosciences·Lauren WalshR Paul Ross
Jun 4, 2021·Biotechnology and Applied Biochemistry·Diego Francisco Benítez-ChaoJosé Rubén Morones-Ramírez

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.