Nuclear magnetic resonance studies of histone IV solution conformation

Biochemistry
A E PekaryT E Wagner

Abstract

The 220-MHz high-resolution proton magnetic resonance (PMR) spectrum of histone IV has been examined as a function of histone concentration, salt concentration, and pD. The hydrophobic C-terminal portion of the histone IV monomer appears to be largely PMR "invisible" indicating that this region of the polypeptide contains rigid secondary structure. Further loss of PMR resonance areas with increased histone IV concentration in neat D2O has been attributed to self-aggregation involving a monomer-dimer equilibrium. An equilibrium between the monomer and large aggregates, on the other hand, appears to dominate at NaCl concentrations above 0.01 M. pD studies reveal an abrupt increase in histone IV aggregation at pD smaller than 0.8 and precipitation of histone IV at pD values in the neighborhood of its isoelectric point, pD similar to 11.

References

Oct 1, 1971·Biochimica Et Biophysica Acta·T A Hoare, E W Johns
Feb 1, 1972·Biopolymers·H J LiI Isenberg
Oct 14, 1969·Journal of Molecular Biology·D Y Tuan, J Bonner
May 11, 1970·Biochemical and Biophysical Research Communications·D G Dearborn, D B Wetlaufer
Sep 1, 1970·The Biochemical Journal·C O Piggott, T G Brady
Jun 22, 1971·Biochemistry·H J LiW C Johnson
Mar 12, 1969·Journal of the American Chemical Society·C C McDonald, W D Phillips
Sep 1, 1969·The Biochemical Journal·P A Ewars, K V Sooter
May 1, 1969·Journal of Theoretical Biology·S Lewin
Oct 15, 1971·FEBS Letters·J H. Diggle, A R. Peacocke
Sep 1, 1957·Biochimica Et Biophysica Acta·N UI
Jul 1, 1958·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·H J CRUFTE STEDMAN

❮ Previous
Next ❯

Citations

May 1, 1979·Die Naturwissenschaften·J Sonnenbichler
Aug 8, 1975·Nucleic Acids Research·H J Li
Apr 1, 1978·Nucleic Acids Research·E Trifonov
Aug 29, 2006·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Xiaodong Wang, Jeffrey J Hayes
Jul 13, 1976·Biochemistry·D E RoarkR L Engle
Jan 1, 1977·International Journal of Peptide and Protein Research·A E Pekary, S I Chan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.