PMID: 6968398Jul 1, 1980Paper

Nuclear magnetic resonance study of the conformation of nucleotides, oligonucleotides, and their analogs in solution. IV. Syn--anti-equilibrium in aqueous solutions of 2'-deoxynucleosides and nucleotides

Molekuliarnaia biologiia
I D BobruskinV L Florent'ev

Abstract

The method of determination of syn-anti-equilibrium constants by experimental measuring of spin-lattice relaxation rates of the H (1') atom of the carbohydrate ring recently elaborated by us for the ribonucleosides and nucleotides was applied to deoxy compounds. For calculation of the contribution of the H (2') and H (2") nuclei rapid relaxation to the relaxation rate of H (1'), the emprical expression was proposed. Conformation situation in aqueous solution of 2'-deoxynucleoside 5'-phosphates and pyrimidine 2'-deoxynucleosides was demonstrated to be characterized by the substantial preponderance of the anti-state. In the solution of dGuo and dAdo the relative weight of the anti-population was decreased to 0.5 and 0.3, respectively. The time average values of glycosidictorsional angle chi in the anti-state was calculated.

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