PMID: 5259755Jul 1, 1969Paper

Nuclear magnetic resonances of reconstituted myoglobins

Proceedings of the National Academy of Sciences of the United States of America
R G ShulmanM Brunori

Abstract

In proton nuclear magnetic resonance spectra of paramagnetic heme proteins, the resonances of the heme groups are shifted away from those of the many hundred protons of the polypeptide chains by interactions with the unpaired electrons and are therefore well resolved at 220 Mc. This paper describes experiments from which these resolved resonances are assigned to specific protons of the heme group in cyanoferrimyoglobin.From a comparison with the proton nuclear magnetic resonance spectra of reconstituted cyanoferrimyoglobins and the corresponding cyanoporphyrin iron (III) complexes, the following groups of heme-protons have been assigned to specific resonances in the NMR spectrum of native cyanoferrimyoglobin: the four ring methyls, the four meso protons, the two -CH groups of the vinyls, and the four methylenes of the propionate side chains. Two resonances of intensity one proton have been assigned to the proximal histidyl residue. The only heme protons whose resonances were not observed are the [unk]CH(2) groups of the vinyl groups which are probably buried in the bulk spectrum of the polypeptide chain. The present data indicate that the protein environment is more important in determining the distribution of unpaired electron...Continue Reading

References

Dec 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·K WüthrichT Yamane
Jun 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·K WüthrichJ Peisach
Mar 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·K WüthrichW S Caughey
Mar 14, 1969·Journal of Molecular Biology·D G DavisC Ho
Dec 1, 1958·Biochimica Et Biophysica Acta·A R FANELLIA CAPUTO

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Citations

Nov 18, 1975·Biochimica Et Biophysica Acta·R J WilliamsJ R Ricard
Nov 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·G M Smith, M D Kamen
Dec 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·G N La MarK C Langry
Dec 31, 1973·Annals of the New York Academy of Sciences·G FeherR Nagel
Jan 1, 1973·Annals of the New York Academy of Sciences·F A Walker, G N La Mar
Sep 16, 1981·Biochemical and Biophysical Research Communications·G N La MarK C Langry
Apr 14, 1971·Journal of Molecular Biology·R G ShulmanM Karplus
Sep 28, 1972·Journal of Molecular Biology·S OgawaT Yamane
Apr 14, 1970·Journal of Molecular Biology·T YamaneS Ogawa
Oct 27, 2018·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·E AndréS Bernad
Nov 24, 1971·Nature: New Biology·L C Dickinson, J C Chien
Jan 19, 2011·Progress in Nuclear Magnetic Resonance Spectroscopy·Peter H J Keizers, Marcellus Ubbink

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