PMID: 9557642Apr 29, 1998Paper

Nuclear matrix attachment regions of human papillomavirus type 16 point toward conservation of these genomic elements in all genital papillomaviruses

Journal of Virology
S H TanHans-Ulrich Bernard

Abstract

The gene functions, transcriptional regulation, and genome replication of human papillomaviruses (HPVs) have been extensively studied. Thus far, however, there has been little research on the organization of HPV genomes in the nuclei of infected cells. As a first step to understand how chromatin and suprachromatin structures may modulate the life cycles of these viruses, we have identified and mapped interactions of HPV DNAs with the nuclear matrix. The endogenous genomes of HPV type 16 (HPV-16) which are present in SiHa, HPKI, and HPKII cells, adhere in vivo to the nuclear matrixes of these cell lines. A tight association with the nuclear matrix in vivo may be common to all genital HPV types, as the genomes of HPV-11, HPV-16, HPV-18, and HPV-33 showed high affinity in vitro to preparations of the nuclear matrix of C33A cells, as did the well-known nuclear matrix attachment region (MAR) of the cellular beta interferon gene. Affinity to the nuclear matrix is not evenly spread over the HPV-16 genome. Five genomic segments have strong MAR properties, while the other parts of the genome have low or no affinity. Some of the five MARs correlate with known cis-responsive elements: a strong MAR lies in the 5' segment of the long contro...Continue Reading

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Citations

Oct 18, 2005·BMC Infectious Diseases·Jeppe VintherBodil Norrild
Jul 30, 2008·Virus Genes·Daniela Marreco CerqueiraCláudia Renata Fernandes Martins
Apr 25, 2012·Asian Pacific Journal of Cancer Prevention : APJCP·Jira ChansaenrojYong Poovorawan
May 12, 2009·Seminars in Cancer Biology·Anita Szalmás, József Kónya

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