Nuclear mean gray level and chromatin distribution changes in cardiomyocytes of heart transplant recipients suffering from acute cellular rejection

Transplantation Proceedings
M ZakliczynskiM Zembala

Abstract

Distribution and staining of the nuclear chromatin are sensitive indicators of changes in physiology and pathology of cells. However, their use in heart transplant recipients is rare. The aim of this study was to compare cardiomyocyte status in heart transplant recipients suffering from moderate acute cellular rejection and subjects without signs of active cellular rejection. One hundred twenty-nine endomyocardial biopsy samples from 43 heart transplant recipients (no later than 6 months after surgery) were evaluated. Overall, 3235 cardiomyocytic nuclei were analyzed using the Quantimet image analysis system to assess the mean gray level: 1584 nuclei were found in biopsies without signs of rejection or with nonsignificant rejection (ISHLT grades 0, 1A, and 1B), whereas the remaining 1651 nuclei were measured from biopsies showing ISHLT grade 3A (significant rejection). Additionally, the chromatin distribution was assessed in all eligible nuclei. The mean gray level was markedly increased in nuclei from biopsy samples with significant rejection (182.6 vs 112.5, P < .001, Mann-Whitney U test). Moreover, the analysis of chromatin distribution revealed significantly more frequent chromatin marginalization and irregular distribution...Continue Reading

References

Apr 1, 1973·American Heart Journal·P K CavesN E Shumway
May 1, 1970·Circulation·C P BieberJ Kosek
Feb 28, 2002·Annual Review of Immunology·Stephen T Smale, Amanda G Fisher
Apr 2, 2003·Regulatory Peptides·Toshihiro TsurudaJohn C Burnett

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Citations

Jun 29, 2017·European Heart Journal Cardiovascular Imaging·B CifraL Mertens
Sep 25, 2012·Circulation. Cardiovascular Imaging·Fatima I LunzeTajinder P Singh

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