Nuclear morphology during the S phase

Microscopy Research and Technique
G MazzottiM Falconi

Abstract

In order to evaluate at the ultrastructural level the chromatin arrangement during the S phase of the cell cycle, the detection of Bromodeoxyuridine (BrdU) by immunogold has been performed in synchronized 3T3 fibroblasts, regenerating liver, and Friend Leukemia Cells (FLC). After a 5-minute BrdU pulse, this label is detected in 10-nm-wide fibers, organized as lacework and assumed to be replication units. In the early part of the S phase, DNA replication units are localized exclusively in the dispersed chromatin domains far from the nuclear envelope. In the middle S, replication occurs at the border between condensed and dispersed chromatin and, finally, in late S, it mainly occurs in perinuclear heterochromatin regions. After replication, the 10-nm fibers can condense in heterochromatin without translocation. Chromatin is highly dispersed in early S and computer image analysis shows an increase in condensed chromatin areas ranging from 13 to 18% at the end of the S phase with a temporal and morphological pattern of distribution characteristic for each cell type. Scanning transmission electron microscopy demonstrates a regular and repetitive structure of dispersed chromatin, represented by a ring-like arrangement of the 10-nm fi...Continue Reading

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Citations

Dec 22, 1999·Archives of Histology and Cytology·P GobbiG Mazzotti
May 15, 2002·Glia·Miguel LafargaJosé G Castaño
Aug 5, 2010·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Laura LagoudakisThierry Tordjmann
Oct 19, 2000·Experimental Cell Research·F JauninS Fakan
Nov 17, 1998·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·G LattanziG Mazzotti
Feb 19, 2008·Developmental Neurobiology·Ana Carolina Dudenhoeffer CarneiroRafael Linden
Aug 5, 2018·Histochemistry and Cell Biology·Irene MasielloMarco Biggiogera

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